G3BP2 sirna

G3BP2 siRNA (Human)

Cat. Num. AAA8202459

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: G3BP2; G3BP2 siRNA (Human); KIAA0660; Ras GTPase-activating protein-binding protein 2; G3BP-2; GAP SH3 domain-binding protein 2; G3BP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: G3BP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 482

• Applicable Applications for G3BP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human G3BP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for G3BP2 sirna

siRNA to inhibit G3BP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19923399
• NCBI GeneID: 9908
• NCBI Accession #: NP_036429.2
• NCBI GenBank Nucleotide #: NM_012297.4
• UniProt Accession #: Q9UN86; O60606; O75149; Q9UPA1; A8K6X1
• Molecular Weight: 50,817 Da
• NCBI Official Full Name: ras GTPase-activating protein-binding protein 2 isoform a
• NCBI Official Synonym Full Names: GTPase activating protein (SH3 domain) binding protein 2
• NCBI Official Symbol: G3BP2
• NCBI Protein Information: ras GTPase-activating protein-binding protein 2
• UniProt Protein Name: Ras GTPase-activating protein-binding protein 2
• Protein Family: Ras GTPase-activating protein-binding protein
• UniProt Gene Name: G3BP2
• UniProt Synonym Gene Names: KIAA0660; G3BP-2
• UniProt Entry Name: G3BP2_HUMAN

Uniprot Description

G3BP-2: an hnRNA-binding protein and an element of the Ras signal transduction pathway. A DNA-unwinding enzyme which can unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. Binds specifically to the Ras-GTPase-activating protein (GAP120) by associating with its SH3 domain. Interacts with USP10, and may regulate it. Several alternatively spliced transcript variants have been described.

Protein type: Adaptor/scaffold; RNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: cytoplasm

Molecular Function: receptor signaling complex scaffold activity; nucleotide binding

Biological Process: cytoplasmic sequestering of NF-kappaB; mRNA transport; Ras protein signal transduction