LHFPL5 sirna

LHFPL5 siRNA (Human)

Cat. Num. AAA8202241

• Gene Names: LHFPL5; TMHS; DFNB67; dJ510O8.8
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LHFPL5; LHFPL5 siRNA (Human); TMHS; Tetraspan membrane protein of hair cell stereocilia; Lipoma HMGIC fusion partner-like 5 protein; LHFPL5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LHFPL5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 219

• Applicable Applications for LHFPL5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LHFPL5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LHFPL5 sirna

siRNA to inhibit LHFPL5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 32698930
• NCBI GeneID: 222662
• NCBI Accession #: NP_872354.1
• NCBI GenBank Nucleotide #: NM_182548.3
• UniProt Accession #: Q8TAF8; B3KX66
• Molecular Weight: 24,201 Da
• NCBI Official Full Name: tetraspan membrane protein of hair cell stereocilia
• NCBI Official Synonym Full Names: lipoma HMGIC fusion partner-like 5
• NCBI Official Symbol: LHFPL5
• NCBI Official Synonym Symbols: TMHS; DFNB67; dJ510O8.8
• NCBI Protein Information: tetraspan membrane protein of hair cell stereocilia
• UniProt Protein Name: Tetraspan membrane protein of hair cell stereocilia
• Protein Family: Tetraspan membrane protein of hair cell stereocilia
• UniProt Gene Name: LHFPL5
• UniProt Synonym Gene Names: TMHS
• UniProt Entry Name: TMHS_HUMAN

NCBI Description

This gene is a member of the lipoma HMGIC fusion partner (LHFP) gene family, which is a subset of the superfamily of tetraspan transmembrane protein encoding genes. Mutations in this gene result in deafness in humans, and a mutation in a similar gene in mice results in deafness and vestibular dysfunction with severe degeneration of the organ of Corti. It is proposed to function in hair bundle morphogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

LHFPL5: May function in hair bundle morphogenesis. Defects in LHFPL5 are a cause of deafness autosomal recessive type 67 (DFNB67). DFNB67 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the LHFP family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: apical plasma membrane; integral to membrane; stereocilium bundle tip

Biological Process: ion transport; auditory receptor cell stereocilium organization and biogenesis; detection of mechanical stimulus involved in sensory perception of sound

Disease: Deafness, Autosomal Recessive 67