ARFGEF1 sirna

ARFGEF1 siRNA (Human)

Cat. Num. AAA8202144

• Gene Names: ARFGEF1; BIG1; P200; ARFGEP1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARFGEF1; ARFGEF1 siRNA (Human); ARFGEP1; BIG1; Brefeldin A-inhibited guanine nucleotide-exchange protein 1; Brefeldin A-inhibited GEP 1; ADP-ribosylation factor guanine nucleotide-exchange factor 1; p200 ARF guanine nucleotide exchange factor; p200 ARF-GEP1; ARFGEF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ARFGEF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1849

• Applicable Applications for ARFGEF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARFGEF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARFGEF1 sirna

siRNA to inhibit ARFGEF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 51479145
• NCBI GeneID: 10565
• NCBI Accession #: NP_006412.2
• NCBI GenBank Nucleotide #: NM_006421.4
• UniProt Accession #: Q9Y6D6; Q9NV46; Q9UFV2; Q9UNL0
• Molecular Weight: 208,767 Da
• NCBI Official Full Name: brefeldin A-inhibited guanine nucleotide-exchange protein 1
• NCBI Official Synonym Full Names: ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)
• NCBI Official Symbol: ARFGEF1
• NCBI Official Synonym Symbols: BIG1; P200; ARFGEP1
• NCBI Protein Information: brefeldin A-inhibited guanine nucleotide-exchange protein 1
• UniProt Protein Name: Brefeldin A-inhibited guanine nucleotide-exchange protein 1
• UniProt Gene Name: ARFGEF1
• UniProt Synonym Gene Names: ARFGEP1; BIG1; Brefeldin A-inhibited GEP 1
• UniProt Entry Name: BIG1_HUMAN

NCBI Description

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Aug 2011]

Uniprot Description

ARFGEF1: Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined.

Protein type: Nucleolus; GEFs; GEFs, ARF

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: small nuclear ribonucleoprotein complex; nucleoplasm; Golgi membrane; Golgi apparatus; nuclear matrix; perinuclear region of cytoplasm; cytoplasm; nucleolus; trans-Golgi network; cytosol

Molecular Function: protein binding; myosin binding; guanyl-nucleotide exchange factor activity; ARF guanyl-nucleotide exchange factor activity

Biological Process: vesicle-mediated transport; protein transport; exocytosis; negative regulation of actin filament polymerization; endomembrane organization; regulation of ARF protein signal transduction; Golgi organization and biogenesis; positive regulation of GTPase activity