PAFAH1B1 sirna

PAFAH1B1 siRNA (Rat)

Cat. Num. AAA8202098

• Gene Names: Pafah1b1; LIS1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PAFAH1B1; PAFAH1B1 siRNA (Rat); LIS-1; LIS1; PAFAHA; Platelet-activating factor acetylhydrolase IB subunit alpha; Lissencephaly-1 protein; PAF acetylhydrolase 45 kDa subunit; PAF-AH 45 kDa subunit; PAF-AH alpha; PAFAH alpha; PAFAH1B1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PAFAH1B1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 410

• Applicable Applications for PAFAH1B1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PAFAH1B1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PAFAH1B1 sirna

siRNA to inhibit PAFAH1B1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13929078
• NCBI GeneID: 83572
• NCBI Accession #: NP_113951.1
• NCBI GenBank Nucleotide #: NM_031763.3
• UniProt Accession #: P63004; O35592; P43035; P81692; Q9R2A6
• Molecular Weight: 46,670 Da
• NCBI Official Full Name: platelet-activating factor acetylhydrolase IB subunit alpha
• NCBI Official Synonym Full Names: platelet-activating factor acetylhydrolase 1b, regulatory subunit 1
• NCBI Official Symbol: Pafah1b1
• NCBI Official Synonym Symbols: LIS1
• NCBI Protein Information: platelet-activating factor acetylhydrolase IB subunit alpha
• UniProt Protein Name: Platelet-activating factor acetylhydrolase IB subunit alpha
• Protein Family: Platelet-activating factor acetylhydrolase
• UniProt Gene Name: Pafah1b1
• UniProt Synonym Gene Names: Lis-1; Lis1; Pafaha; LIS-1; PAF-AH 45 kDa subunit; PAFAH alpha
• UniProt Entry Name: LIS1_RAT

NCBI Description

subunit of the brain intracellular platelet-activating factor acetylhydrolase, which is composed of alpha1, alpha2, and beta subunits [RGD, Feb 2006]

Uniprot Description

PAFAH1B1: Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet- activating factor (PAF) by removing the acetyl group at the SN-2 position. Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Defects in PAFAH1B1 are the cause of lissencephaly type 1 (LIS1); also known as classic lissencephaly. LIS1 is characterized by agyria or pachgyria and disorganization of the clear neuronal lamination of normal six-layered cortex. The cortex is abnormally thick and poorly organized with 4 primitive layers. LIS1 is associated with enlarged and dysmorphic ventricles and often hypoplasia of the corpus callosum. Defects in PAFAH1B1 are the cause of subcortical band heterotopia (SBH). SBH is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric ribbons of gray matter found in the central white matter between the cortex and the ventricular surface. Defects in PAFAH1B1 are a cause of Miller-Dieker lissencephaly syndrome (MDLS). MDLS is a contiguous gene deletion syndrome of chromosome 17p13.3, characterized by classical lissencephaly and distinct facial features. Additional congenital malformations can be part of the condition. Belongs to the WD repeat LIS1/nudF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - ether lipid; Cell cycle regulation

Cellular Component: astral microtubule; centrosome; neuron projection; nuclear membrane; nonmotile primary cilium; cell; leading edge; nuclear envelope; cell cortex; cytosol; microtubule cytoskeleton; kinetochore; kinesin complex; microtubule associated complex; growth cone; cell soma; perinuclear region of cytoplasm; axon; cytoplasm; motile primary cilium; intracellular; vesicle

Molecular Function: dynein binding; protein binding; protein homodimerization activity; platelet-activating factor acetyltransferase activity; microtubule binding; protein complex binding; dynein intermediate chain binding; phosphoprotein binding

Biological Process: acrosome formation; negative regulation of JNK cascade; adult locomotory behavior; stem cell division; positive regulation of axon extension; protein secretion; neuron migration; positive regulation of mitotic cell cycle; retrograde axon cargo transport; microtubule-based process; cerebral cortex neuron differentiation; synaptic transmission; establishment of centrosome localization; learning and/or memory; establishment of mitotic spindle orientation; brain morphogenesis; vesicle transport along microtubule; neuromuscular process controlling balance; layer formation in the cerebral cortex; microtubule organizing center organization and biogenesis; mitosis; cell migration; corpus callosum morphogenesis; hippocampus development; nuclear envelope disassembly; transmission of nerve impulse; microtubule cytoskeleton organization and biogenesis; ameboidal cell migration; neuroblast proliferation; brain development; cerebral cortex development; actin cytoskeleton organization and biogenesis; positive regulation of cytokine and chemokine mediated signaling pathway; nuclear migration; lipid catabolic process