RDH5 sirna

RDH5 siRNA (Human)

Cat. Num. AAA8201959

• Gene Names: RDH5; RDH1; 9cRDH; SDR9C5; HSD17B9
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RDH5; RDH5 siRNA (Human); RDH1; 11-cis retinol dehydrogenase; 11-cis RDH; 11-cis RoDH; 9-cis retinol dehydrogenase; 9cRDH; RDH5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RDH5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 318

• Applicable Applications for RDH5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RDH5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RDH5 sirna

siRNA to inhibit RDH5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 315113906
• NCBI GeneID: 5959
• NCBI Accession #: NP_001186700.1
• NCBI GenBank Nucleotide #: NM_001199771.1
• UniProt Accession #: Q92781; O00179; Q8TAI2
• Molecular Weight: 34,979 Da
• NCBI Official Full Name: 11-cis retinol dehydrogenase
• NCBI Official Synonym Full Names: retinol dehydrogenase 5 (11-cis/9-cis)
• NCBI Official Symbol: RDH5
• NCBI Official Synonym Symbols: RDH1; 9cRDH; SDR9C5; HSD17B9
• NCBI Protein Information: 11-cis retinol dehydrogenase
• UniProt Protein Name: 11-cis retinol dehydrogenase
• Protein Family: 11-cis retinol dehydrogenase
• UniProt Gene Name: RDH5
• UniProt Synonym Gene Names: HSD17B9; RDH1; SDR9C5; 11-cis RDH; 11-cis RoDH; 9cRDH
• UniProt Entry Name: RDH1_HUMAN

NCBI Description

This gene encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments. Mutations in this gene cause autosomal recessive fundus albipunctatus, a rare form of night blindness that is characterized by a delay in the regeneration of cone and rod photopigments. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream BLOC1S1 (biogenesis of lysosomal organelles complex-1, subunit 1) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

RDH5: Stereospecific 11-cis retinol dehydrogenase, which catalyzes the final step in the biosynthesis of 11-cis retinaldehyde, the universal chromophore of visual pigments. Also able to oxidize 9-cis-retinol and 13-cis-retinol, but not all- trans-retinol. Active in the presence of NAD as cofactor but not in the presence of NADP. Defects in RDH5 are a cause of retinitis punctata albescens (RPA); also known as fundus albipunctatus (FA). RPA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: EC 1.1.1.315; Oxidoreductase; Endoplasmic reticulum; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 12q13-q14

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum lumen

Molecular Function: retinol dehydrogenase activity

Biological Process: phototransduction, visible light; visual perception; retinol metabolic process; retinoid metabolic process

Disease: Fundus Albipunctatus