PTF1A sirna

PTF1A siRNA (Mouse)

Cat. Num. AAA8201531

• Gene Names: Ptf1a; PTF1p48; bHLHa29; PTF1-p48
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTF1A; PTF1A siRNA (Mouse); PTF1P48; Pancreas transcription factor 1 subunit alpha; Pancreas-specific transcription factor 1a; bHLH transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1; PTF1-p48; PTF1A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PTF1A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 324

• Applicable Applications for PTF1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PTF1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTF1A sirna

siRNA to inhibit PTF1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 224967077
• NCBI GeneID: 19213
• NCBI Accession #: NP_061279.2
• NCBI GenBank Nucleotide #: NM_018809.2
• UniProt Accession #: Q9QX98; Q9QYF5; Q9QYF6
• Molecular Weight: 35,185 Da
• NCBI Official Full Name: pancreas transcription factor 1 subunit alpha
• NCBI Official Synonym Full Names: pancreas specific transcription factor, 1a
• NCBI Official Symbol: Ptf1a
• NCBI Official Synonym Symbols: PTF1p48; bHLHa29; PTF1-p48
• NCBI Protein Information: pancreas transcription factor 1 subunit alpha
• UniProt Protein Name: Pancreas transcription factor 1 subunit alpha
• Protein Family: Pancreas transcription factor
• UniProt Gene Name: Ptf1a
• UniProt Synonym Gene Names: Ptf1p48; PTF1-p48
• UniProt Entry Name: PTF1A_MOUSE

Uniprot Description

PTF1A: Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH).

Cellular Component: transcription factor complex; cytoplasm; intracellular; nucleus

Molecular Function: protein dimerization activity; protein binding; DNA binding; sequence-specific DNA binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; retinoic acid receptor signaling pathway; tissue development; nervous system development; cell fate commitment; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; multicellular organismal development; neuron fate commitment; exocrine pancreas development; embryonic development; regulation of transcription, DNA-dependent; generation of neurons; pancreas development; cerebellum development; positive regulation of transcription from RNA polymerase II promoter; cell differentiation; retina morphogenesis in camera-type eye; hindbrain development