FLCN sirna

FLCN siRNA (Human)

Cat. Num. AAA8201345

• Gene Names: FLCN; BHD; FLCL
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FLCN; FLCN siRNA (Human); BHD; Folliculin; BHD skin lesion fibrofolliculoma protein; Birt-Hogg-Dube syndrome protein; FLCN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FLCN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 342

• Applicable Applications for FLCN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FLCN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FLCN sirna

siRNA to inhibit FLCN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21389403
• NCBI GeneID: 201163
• NCBI Accession #: NP_653207.1
• NCBI GenBank Nucleotide #: NM_144606.5
• UniProt Accession #: Q8NFG4; Q6ZRX1; Q96BD2; Q96BE4; A6NJJ8
• Molecular Weight: 20,877 Da
• NCBI Official Full Name: folliculin isoform 2
• NCBI Official Synonym Full Names: folliculin
• NCBI Official Symbol: FLCN
• NCBI Official Synonym Symbols: BHD; FLCL
• NCBI Protein Information: folliculin
• UniProt Protein Name: Folliculin
• Protein Family: Folliculin
• UniProt Gene Name: FLCN
• UniProt Synonym Gene Names: BHD
• UniProt Entry Name: FLCN_HUMAN

NCBI Description

This gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene are associated with Birt-Hogg-Dube syndrome, which is characterized by fibrofolliculomas, renal tumors, lung cysts, and pneumothorax. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FLCN: a protein that is expressed in most tissues and that has no significant homology to other proteins. Involved in nutrient sensing through the AMPK and mTOR signaling pathways. Regulates lysosome function by promoting the mTORC1-dependent sequestration of transcription factor EB (TFEB). Required for the recruitment of mTORC1 to lysosomes by Rag GTPases. Amino acid depletion leads to its recruitment to the surface of lysosomes where it directly binds RagA, an interaction that is promoted by FNIP1. May be a tumor suppressor. Loss of function mutations cause Birt-Hogg-Dube syndrome (BHD), a disease characterized by fibrofolliculomas (hair follicle tumors), and predispositions for renal carcinoma, pneumothorax, and pulmonary cysts. These symptoms are similar to those that occur in TSC or tuberous sclerosis complex, suggesting the involvement in the same pathways as TSC1. Belongs to the folliculin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cytoplasm; plasma membrane; midbody; nucleus

Molecular Function: protein binding; protein complex binding

Biological Process: intercellular junction assembly; regulation of protein amino acid phosphorylation; negative regulation of TOR signaling pathway; TOR signaling pathway; negative regulation of protein kinase B signaling cascade; positive regulation of cell adhesion; in utero embryonic development; positive regulation of apoptosis; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of histone acetylation; negative regulation of transcription from RNA polymerase II promoter; regulation of cytokinesis; regulation of TOR signaling pathway; negative regulation of Rho protein signal transduction; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; negative regulation of cell growth; negative regulation of cell migration; positive regulation of TOR signaling pathway

Disease: Birt-hogg-dube Syndrome; Pneumothorax, Primary Spontaneous; Colorectal Cancer; Renal Cell Carcinoma, Nonpapillary