KLF10 sirna

KLF10 siRNA (Human)

Cat. Num. AAA8201241

• Gene Names: KLF10; EGRA; TIEG; TIEG1; EGR-alpha
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KLF10; KLF10 siRNA (Human); TIEG; TIEG1; Krueppel-like factor 10; EGR-alpha; Transforming growth factor-beta-inducible early growth response protein 1; TGFB-inducible early growth response protein 1; TIEG-1; KLF10 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KLF10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 123

• Applicable Applications for KLF10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KLF10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KLF10 sirna

siRNA to inhibit KLF10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 73760403
• NCBI GeneID: 7071
• NCBI Accession #: NP_001027453.1
• NCBI GenBank Nucleotide #: NM_001032282.3
• UniProt Accession #: Q13118; O75411; Q503B2; A8MVH0; B2R794; L0R4P6; L0R679
• Molecular Weight: 13,702 Da
• NCBI Official Full Name: Krueppel-like factor 10 isoform b
• NCBI Official Synonym Full Names: Kruppel-like factor 10
• NCBI Official Symbol: KLF10
• NCBI Official Synonym Symbols: EGRA; TIEG; TIEG1; EGR-alpha
• NCBI Protein Information: Krueppel-like factor 10
• UniProt Protein Name: Krueppel-like factor 10
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF10
• UniProt Synonym Gene Names: TIEG; TIEG1; TGFB-inducible early growth response protein 1; TIEG-1
• UniProt Entry Name: KLF10_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that feature C2H2-type zinc finger domains. The encoded protein is a transcriptional repressor that acts as an effector of transforming growth factor beta signaling. Activity of this protein may inhibit the growth of cancers, particularly pancreatic cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

KLF10: Transcriptional repressor involved in the regulation of cell growth. Inhibits cell growth. Binds to the consensus sequence 5'-GGTGTG-3'. Belongs to the Sp1 C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: nucleus

Molecular Function: protein binding; metal ion binding; transcription factor activity

Biological Process: circadian rhythm; negative regulation of cell proliferation; cell proliferation; cell-cell signaling; transcription, DNA-dependent; positive regulation of osteoclast differentiation; somatic stem cell maintenance; transforming growth factor beta receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; regulation of circadian rhythm; negative regulation of transcription, DNA-dependent; cellular response to starvation; skeletal development; bone mineralization