DNM1L sirna

DNM1L siRNA (Mouse)

Cat. Num. AAA8201236

• Gene Names: Dnm1l; Dlp1; Drp1; Dnmlp1; python; AI450666; 6330417M19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DNM1L; DNM1L siRNA (Mouse); DRP1; Dynamin-1-like protein; Dynamin family member proline-rich carboxyl-terminal domain less; Dymple; Dynamin-related protein 1; DNM1L sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DNM1L siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 221

• Applicable Applications for DNM1L sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DNM1L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DNM1L sirna

siRNA to inhibit DNM1L expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 71061455
• NCBI GeneID: 74006
• NCBI Accession #: NP_001021118.1
• NCBI GenBank Nucleotide #: NM_001025947.2
• UniProt Accession #: Q8K1M6; Q8BNQ5; Q8BQ64; Q8CGD0; Q8K1A1
• Molecular Weight: 24,488 Da
• NCBI Official Full Name: dynamin-1-like protein isoform b
• NCBI Official Synonym Full Names: dynamin 1-like
• NCBI Official Symbol: Dnm1l
• NCBI Official Synonym Symbols: Dlp1; Drp1; Dnmlp1; python; AI450666; 6330417M19Rik
• NCBI Protein Information: dynamin-1-like protein
• UniProt Protein Name: Dynamin-1-like protein
• Protein Family: Dynamin-1-like protein
• UniProt Gene Name: Dnm1l
• UniProt Synonym Gene Names: Drp1; Dymple
• UniProt Entry Name: DNM1L_MOUSE

NCBI Description

This gene encodes a member of the dynamin family. The encoded protein is localized to the cytoplasm and mitochondrial membrane, is involved in mitochondrial and peroxisomal division, and is essential for mitochondrial fission. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 2. [provided by RefSeq, Feb 2013]

Uniprot Description

DRP1: Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis- dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport. Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Can self-assemble in multimeric ring-like structures. Interacts with BCL2L1; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with FIS1. Interacts with GSK3B and MARCH5. Interacts (via the GTPase and B domains) with UBE2I; the interaction promotes sumoylation of DNM1L, mainly in ite B domain. Interacts with PPP3CA; the interaction dephosphorylates DNM1L and regulates its transition to mitochondria. Interacts witn MID49 and MID51. Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. Belongs to the dynamin family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.5.5; Microtubule-binding; Motor; Hydrolase; Apoptosis; Endoplasmic reticulum; Mitochondrial

Cellular Component: Golgi apparatus; microtubule; protein complex; intracellular membrane-bound organelle; mitochondrion; coated pit; cytosol; mitochondrial outer membrane; membrane; perinuclear region of cytoplasm; cytoplasm; peroxisome; synapse; cytoplasmic vesicle; cell junction

Molecular Function: GTPase activity; identical protein binding; protein binding; protein homodimerization activity; GTP binding; hydrolase activity; ubiquitin protein ligase binding; nucleotide binding; lipid binding

Biological Process: peroxisome fission; mitochondrial fission; release of cytochrome c from mitochondria; positive regulation of protein secretion; metabolic process; positive regulation of apoptosis; programmed cell death; regulation of protein oligomerization; endocytosis; mitochondrial fragmentation during apoptosis; protein homotetramerization