MYO15A sirna

MYO15A siRNA (Human)

Cat. Num. AAA8201162

• Gene Names: MYO15A; DFNB3; MYO15
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYO15A; MYO15A siRNA (Human); MYO15; Unconventional myosin-XV; Unconventional myosin-15; MYO15A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MYO15A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 797

• Applicable Applications for MYO15A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MYO15A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYO15A sirna

siRNA to inhibit MYO15A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 118402590
• NCBI GeneID: 51168
• NCBI Accession #: NP_057323.3
• NCBI GenBank Nucleotide #: NM_016239.3
• UniProt Accession #: Q9UKN7; B4DFC7
• Molecular Weight: 88,988 Da
• NCBI Official Full Name: unconventional myosin-XV
• NCBI Official Synonym Full Names: myosin XVA
• NCBI Official Symbol: MYO15A
• NCBI Official Synonym Symbols: DFNB3; MYO15
• NCBI Protein Information: unconventional myosin-XV
• UniProt Protein Name: Unconventional myosin-XV
• Protein Family: Unconventional myosin
• UniProt Gene Name: MYO15A
• UniProt Synonym Gene Names: MYO15
• UniProt Entry Name: MYO15_HUMAN

NCBI Description

This gene encodes an unconventional myosin. This protein differs from other myosins in that it has a long N-terminal extension preceding the conserved motor domain. Studies in mice suggest that this protein is necessary for actin organization in the hair cells of the cochlea. Mutations in this gene have been associated with profound, congenital, neurosensory, nonsyndromal deafness. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Read-through transcripts containing an upstream gene and this gene have been identified, but they are not thought to encode a fusion protein. Several alternatively spliced transcript variants have been described, but their full length sequences have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

MYO15A: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. Required for the arrangement of stereocilia in mature hair bundles. Defects in MYO15A are the cause of deafness autosomal recessive type 3 (DFNB3). DFNB3 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: stereocilium; cytoplasm; myosin complex

Molecular Function: calmodulin binding; motor activity; actin binding; ATP binding

Biological Process: inner ear morphogenesis; sensory perception of sound; metabolic process; locomotory behavior

Disease: Deafness, Autosomal Recessive 3