H2-EA sirna

H2-EA siRNA (Mouse)

Cat. Num. AAA8201145

• Gene Names: H2-Ea-ps; Ia3; Ia-3; H-2Ea; H2-Ea; AI323765; I-Ealpha; E-alpha-f; MHC-H2-Ea
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: H2-EA; H2-EA siRNA (Mouse); H2-EA-PS; H-2 class II histocompatibility antigen E-U alpha chain; H2-EA sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: H2-EA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 255

• Applicable Applications for H2-EA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse H2-EA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for H2-EA sirna

siRNA to inhibit H2-EA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31982789
• NCBI GeneID: 100504404
• NCBI Accession #: NP_034511.2
• NCBI GenBank Nucleotide #: NM_010381.2
• UniProt Accession #: P14439; Q31092; P01904; P04224
• Molecular Weight: 29,108 Da
• NCBI Official Full Name: histocompatibility 2, class II antigen E alpha
• NCBI Official Synonym Full Names: histocompatibility 2, class II antigen E alpha, pseudogene
• NCBI Official Symbol: H2-Ea-ps
• NCBI Official Synonym Symbols: Ia3; Ia-3; H-2Ea; H2-Ea; AI323765; I-Ealpha; E-alpha-f; MHC-H2-Ea
• NCBI Protein Information: histocompatibility 2, class II antigen E alpha
• UniProt Protein Name: H-2 class II histocompatibility antigen, E-U alpha chain
• Protein Family: H-2 class II histocompatibility antigen
• UniProt Gene Name: H2-Ea
• UniProt Synonym Gene Names: H2-Ea-ps
• UniProt Entry Name: HA23_MOUSE

NCBI Description

This locus belongs to the class II major histocompatibility complex (MHC) family of genes, which encode immune response (Ia) antigens that function in the T-cell-dependent immune response. This family member has multiple haplotypes, some of which result in the production of an E-alpha subunit that combines with an E-beta subunit to form a functional E complex at the cell surface. Other haplotypes, including that of the reference genome allele, contain mutations and they thus represent polymorphic pseudogenes that do not produce functional products. These mutations include frameshifting indels, nonsense mutations, and deletions of larger regions. The reference genome haplotype contains a deletion at the 5' end of the gene, including the core promoter region and the transcription start site, and therefore no transcripts result from this haplotype. [provided by RefSeq, Aug 2011]