CP sirna

CP siRNA (Mouse)

Cat. Num. AAA8200924

• Gene Names: Cp; D3Ertd555e
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CP; CP siRNA (Mouse); Ceruloplasmin; Ferroxidase; CP sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,061

• Applicable Applications for CP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CP sirna

siRNA to inhibit CP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 442745912
• NCBI GeneID: 12870
• NCBI Accession #: NP_001263177.1
• NCBI GenBank Nucleotide #: NM_001276248.1
• UniProt Accession #: Q61147; Q6P5C8
• Molecular Weight: 121,151 Da
• NCBI Official Full Name: ceruloplasmin isoform c
• NCBI Official Synonym Full Names: ceruloplasmin
• NCBI Official Symbol: Cp
• NCBI Official Synonym Symbols: D3Ertd555e
• NCBI Protein Information: ceruloplasmin
• UniProt Protein Name: Ceruloplasmin
• Protein Family: Ceruloplasmin
• UniProt Gene Name: Cp
• UniProt Entry Name: CERU_MOUSE

NCBI Description

The protein encoded by this gene is a copper-containing glycoprotein found soluble in the serum and GPI-anchored in other tissues. It oxidizes Fe(II) to Fe(III) and is proposed to play an important role in iron homeostasis. In humans mutations of this gene cause aceruloplasminemia, which is characterized by retinal degeneration, diabetes, anemia and neurological symptoms. In mouse deficiency of this gene in combination with a deficiency of its homolog hephaestin causes retinal degeneration and serves as a pathophysiological model for aceruloplasminemia and age-related macular degeneration. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Uniprot Description

CP: Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense. Defects in CP are the cause of aceruloplasminemia (ACERULOP). It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances. Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. Belongs to the multicopper oxidase family.

Protein type: Oxidoreductase; Secreted, signal peptide; EC 1.16.3.1; Secreted; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Cellular Component: extracellular space; anchored to plasma membrane; lysosomal membrane; extracellular region

Molecular Function: ferroxidase activity; copper ion binding; metal ion binding; chaperone binding; oxidoreductase activity

Biological Process: response to copper ion; cellular iron ion homeostasis; transport; ion transport; copper ion transport