GRIN2C sirna

GRIN2C siRNA (Rat)

Cat. Num. AAA8200837

• Gene Names: Grin2c; NR2C; GluN2C
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GRIN2C; GRIN2C siRNA (Rat); Glutamate receptor ionotropic. NMDA 2C; GluN2C; Glutamate [NMDA] receptor subunit epsilon-3; N-methyl D-aspartate receptor subtype 2C; NMDAR2C; NR2C; GRIN2C sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GRIN2C siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1250

• Applicable Applications for GRIN2C sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GRIN2C gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GRIN2C sirna

siRNA to inhibit GRIN2C expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148747563
• NCBI GeneID: 24411
• NCBI Accession #: NP_036707.3
• NCBI GenBank Nucleotide #: NM_012575.3
• UniProt Accession #: Q00961
• Molecular Weight: 135,271 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2C
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 2C
• NCBI Official Symbol: Grin2c
• NCBI Official Synonym Symbols: NR2C; GluN2C
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2C
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2C
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: Grin2c
• UniProt Synonym Gene Names: GluN2C; NMDAR2C; NR2C
• UniProt Entry Name: NMDE3_RAT

NCBI Description

subunit that in combination with NMDAR1 displays NMDA receptor activity; may contribute to NMDA receptor functional diversity [RGD, Feb 2006]

Uniprot Description

NMDAR2C: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily.

Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral; Channel, calcium

Cellular Component: postsynaptic membrane; dendrite; postsynaptic density; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; N-methyl-D-aspartate selective glutamate receptor activity; protein N-terminus binding; cation channel activity; PDZ domain binding

Biological Process: synaptic transmission, glutamatergic; regulation of membrane potential; protein localization; startle response; ionotropic glutamate receptor signaling pathway; directional locomotion; response to wounding; regulation of excitatory postsynaptic membrane potential; neuromuscular process controlling balance; negative regulation of protein catabolic process