PAX4 sirna

PAX4 siRNA (Human)

Cat. Num. AAA8200617

• Gene Names: PAX4; KPD; MODY9
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PAX4; PAX4 siRNA (Human); Paired box protein Pax-4; PAX4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PAX4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 343

• Applicable Applications for PAX4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PAX4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PAX4 sirna

siRNA to inhibit PAX4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 170784824
• NCBI GeneID: 5078
• NCBI Accession #: NP_006184.2
• NCBI GenBank Nucleotide #: NM_006193.2
• UniProt Accession #: O43316; O95161; Q6B0H0
• Molecular Weight: 37,061 Da
• NCBI Official Full Name: paired box protein Pax-4
• NCBI Official Synonym Full Names: paired box 4
• NCBI Official Symbol: PAX4
• NCBI Official Synonym Symbols: KPD; MODY9
• NCBI Protein Information: paired box protein Pax-4
• UniProt Protein Name: Paired box protein Pax-4
• Protein Family: Paired box protein
• UniProt Gene Name: PAX4
• UniProt Entry Name: PAX4_HUMAN

NCBI Description

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The paired box 4 gene is involved in pancreatic islet development and mouse studies have demonstrated a role for this gene in differentiation of insulin-producing beta cells. [provided by RefSeq, Jul 2008]

Uniprot Description

PAX4: Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that binds to a common element in the glucagon, insulin and somatostatin promoters. Competes with PAX6 for this same promoter binding site. Isoform 2 appears to be a dominant negative form antagonizing PAX4 transcriptional activity. Defects in PAX4 are a cause of noninsulin-dependent diabetes mellitus (NIDDM); also known as diabetes mellitus type 2 or maturity-onset diabetes. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. Genetic variations in PAX4 are associated with susceptibility to insulin-dependent diabetes mellitus (IDDM). IDDM normally starts in childhood or adolescence and is caused by the body's own immune system which destroys the insulin-producing beta cells in the pancreas. Classical features are polydipsia, polyphagia and polyuria, due to hyperglycemia- induced osmotic diuresis. Defects in PAX4 are a cause of susceptibility to diabetes mellitus ketosis-prone (KPD). KPD is an atypical form of diabetes mellitus characterized by an acute initial presentation with severe hyperglycemia and ketosis, as seen in classic type 1 diabetes, but after initiation of insulin therapy, prolonged remission is often possible with cessation of insulin therapy and maintenance of appropriate metabolic control. Metabolic studies show a markedly blunted insulin secretory response to glucose, partially reversible with the improvement of blood glucose control. Variable levels of insulin resistance are observed, especially in obese patients. Pancreatic beta-cell autoimmunity is a rare finding. Defects in PAX4 are the cause of maturity-onset diabetes of the young type 9 (MODY9). MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Belongs to the paired homeobox family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: nucleoplasm

Molecular Function: DNA binding; double-stranded DNA binding

Biological Process: response to drug; circadian rhythm; organ morphogenesis; response to cAMP; transcription, DNA-dependent; retina development in camera-type eye; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; cell differentiation; endocrine pancreas development; negative regulation of apoptosis

Disease: Maturity-onset Diabetes Of The Young, Type 9; Diabetes Mellitus, Ketosis-prone; Diabetes Mellitus, Noninsulin-dependent