WISP3 sirna

WISP3 siRNA (Mouse)

Cat. Num. AAA8200206

• Gene Names: Wisp3; CCN6; Gm735; WISP-3; ENSMUSG00000062074
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WISP3; WISP3 siRNA (Mouse); CCN6; WNT1-inducible-signaling pathway protein 3; WISP-3; CCN family member 6; WISP3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WISP3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 354

• Applicable Applications for WISP3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WISP3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WISP3 sirna

siRNA to inhibit WISP3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 188497673
• NCBI GeneID: 327743
• NCBI Accession #: NP_001120848.1
• NCBI GenBank Nucleotide #: NM_001127376.1
• UniProt Accession #: D3Z5L9
• Molecular Weight: 39,379 Da
• NCBI Official Full Name: WNT1-inducible-signaling pathway protein 3
• NCBI Official Synonym Full Names: WNT1 inducible signaling pathway protein 3
• NCBI Official Symbol: Wisp3
• NCBI Official Synonym Symbols: CCN6; Gm735; WISP-3; ENSMUSG00000062074
• NCBI Protein Information: WNT1-inducible-signaling pathway protein 3
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 3
• Protein Family: WNT1-inducible-signaling pathway protein
• UniProt Gene Name: Wisp3
• UniProt Synonym Gene Names: Ccn6; WISP-3
• UniProt Entry Name: WISP3_MOUSE

Uniprot Description

WISP3: Appears to be required for normal postnatal skeletal growth and cartilage homeostasis. Defects in WISP3 are the cause of progressive pseudorheumatoid arthropathy of childhood (PPAC). PPAC is an autosomal recessive disorder characterized by stiffness and swelling of joints, motor weakness and joint contractures. Signs and symptoms of the disease develop typically between three and eight years of age. This progressive disease is a primary disorder of articular cartilage with continued cartilage loss and destructive bone changes with aging. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell cycle regulation

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; growth factor activity

Biological Process: negative regulation of cell proliferation; negative regulation of angiogenesis; cell-cell signaling; regulation of cell growth; cell adhesion; signal transduction