SPAG9 sirna

SPAG9 siRNA (Mouse)

Cat. Num. AAA8200094

• Gene Names: Spag9; JLP; Jip4; syd1; JSAP2; JSAP2a; AW552012; Mapk8ip4; 3110018C07Rik; 4733401I23Rik; 4831406C20Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPAG9; SPAG9 siRNA (Mouse); JIP4; JSAP2; KIAA0516; MAPK8IP4; C-Jun-amino-terminal kinase-interacting protein 4; JIP-4; JNK-interacting protein 4; JNK-associated leucine-zipper protein; JLP; JNK/SAPK-associated protein 2; Mitogen-activated protein kinase 8-interacting protein 4; Sperm-associated antigen 9; SPAG9 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SPAG9 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1169

• Applicable Applications for SPAG9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SPAG9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPAG9 sirna

siRNA to inhibit SPAG9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 70887778
• NCBI GeneID: 70834
• NCBI Accession #: NP_001020599.1
• NCBI GenBank Nucleotide #: NM_001025428.1
• UniProt Accession #: Q58A65; Q3UH77; Q3UHF0; Q58VQ4; Q5NC70; Q5NC78; Q6A057; Q6PAS3; Q8CJC2
• Molecular Weight: 125,350 Da
• NCBI Official Full Name: C-Jun-amino-terminal kinase-interacting protein 4 isoform 2
• NCBI Official Synonym Full Names: sperm associated antigen 9
• NCBI Official Symbol: Spag9
• NCBI Official Synonym Symbols: JLP; Jip4; syd1; JSAP2; JSAP2a; AW552012; Mapk8ip4; 3110018C07Rik; 4733401I23Rik; 4831406C20Rik
• NCBI Protein Information: C-Jun-amino-terminal kinase-interacting protein 4
• UniProt Protein Name: C-Jun-amino-terminal kinase-interacting protein 4
• Protein Family: C-Jun-amino-terminal kinase-interacting protein
• UniProt Gene Name: Spag9
• UniProt Synonym Gene Names: Jip4; Jsap2; Kiaa0516; Mapk8ip4; JIP-4; JNK-interacting protein 4; JLP; JSAP2
• UniProt Entry Name: JIP4_MOUSE

Uniprot Description

JIP4: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg- interaction. Homooligomer. Interacts with MAX, MAPK8, MAPK9, MAPK10, MAPK14, MAP3K3, MYC, KNS2 and MAP2K4. Interaction with KNS2 is important in the formation of ternary complex with MAPK8. Interacts with NFKB1. Isoform 3 is increased in systemic sclerosis fibroblasts. Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis. Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients. Belongs to the JIP scaffold family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Cancer Testis Antigen (CTA)

Cellular Component: perinuclear region of cytoplasm; cytoplasm

Molecular Function: JUN kinase binding; protein binding; MAP-kinase scaffold activity; mitogen-activated protein kinase p38 binding; receptor signaling complex scaffold activity; kinesin binding

Biological Process: vesicle-mediated transport; positive regulation of signal transduction; positive regulation of MAPKKK cascade; activation of MAPK activity; striated muscle cell differentiation; positive regulation of neuron differentiation; retrograde transport, endosome to Golgi; protein homooligomerization; activation of JNK activity; positive regulation of cell migration