CD59 recombinant protein

CD59 Protein, Cynomolgus, Recombinant (His Tag)

Cat. Num. AAA8123572

• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD59; CD59 Protein; Cynomolgus; Recombinant (His Tag); Cynomolgus CD59/CD59A/MAC-IP Protein (His Tag); CD59 molecule; CD59 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu101

• Species: Cynomolgus
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 26
• Tag: C-His
• Protein Construction: A DNA sequence encoding the cynomolgus CD59 (G7PQF7)(Met1-Glu101) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD59 recombinant protein

Background: CD59 glycoprotein, also known as 2kDa homologous restriction factor, HRF2, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

Product Categories/Family for CD59 recombinant protein

Complement Activation Pathways

References

Fletcher CM. et al., 1994, Structure. 2: 185-99. Rudd PM. et al., 1997, J Biol Chem. 272: 7229-44. Kimberley FC. et al., 2007, Mol Immunol. 44 (1-3): 73-81. Gong Y. et al., 2007, Sci China C Life Sci. 50 (6): 773-9. Picariello G. et al., 2008, Proteomics 8: 3833-47. Heibeck TH. et al., 2009, J Proteome Res. 8: 3852-61.

NCBI and Uniprot Product Information

• UniProt Accession #: G7PQF7
• Molecular Weight: The recombinant cynomolgus CD59 comprises 87 amino acids and has a calculated molecular mass of 10.2kDa. The apparent molecular mass of it is approximately 18 and 14kDa respectively in SDS-PAGE.
• Protein Family: CD59 glycoprotein