Ephrin B2 Active Protein | EFNB2 active protein

Ephrin B2 Protein, Canine, Recombinant (hFc Tag)

Cat. Num. AAA8123313

• Purity: >95% as determined by SDS-PAGE
• Synonyms: Ephrin B2; Ephrin B2 Protein; Canine; Recombinant (hFc Tag); Canine Ephrin-B2/EFNB2 Protein (Fc Tag); ephrin-B2; EFNB2 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala229

• Species: Canine
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ile 28
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the canine EFNB2 (B0LDS6)(Met1-Ala229) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized human EphB4-His at 10ug/mL (100uL/well) can bind canine EFNB2-Fc3. The EC50 of canine EFNB2-Fc3 is 25.8-60.2ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human EphB4-His at 10 ug/mL (100 uL/well) can bind canine EFNB2-Fc3. The EC50 of canine EFNB2-Fc3 is 25.8-60.2 ng/mL.)

Related Product Information for EFNB2 active protein

Background: EphrinB2 also known as EFNB2 is a member of the ephrin family. EphrinB2 is involved in establishing arterial versus venous identity and perhaps in anastamosing arterial and venous vessels at their junctions. The transmembrane-associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB2 expression progressively extends from the arterial endothelium to surrounding smooth muscle cells and to pericytes, suggesting that ephrin-B2 may play an important role during formation of the arterial muscle wall.

References

Wang HU, et al. (1998) Molecular distinction and angiogenic interaction between embryonic arteries and veins revealed by ephrin-B2 and its receptor Eph-B4. Cell. 93(5): 741-53.Gale NW, et al. (2001) Ephrin-B2 selectively marks arterial vessels and neovascularization sites in the adult, with expression in both endothelial and smooth-muscle cells. Dev Biol. 230(2): 151-60.Shin D, et al. (2001) Expression of ephrinB2 identifies a stable genetic difference between arterial and venous vascular smooth muscle as well as endothelial cells, and marks subsets of microvessels at sites of adult neovascularization. Dev Biol. 230(2): 139-50.

NCBI and Uniprot Product Information

• UniProt Accession #: B0LDS6
• Molecular Weight: The recombinant canine EFNB2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 443 amino acids and has a predicted molecular mass of 49.2kDa. The apparent molecular mass of the protein is approximately 58 and 35kDa in SDS-PAGE under reduci
• Protein Family: Ephrin