TSHR recombinant protein

TSHR Protein, Human, Recombinant (ECD, His Tag)

Cat. Num. AAA8122096

• Gene Names: TSHR; LGR3; CHNG1; hTSHR-I
• Purity: >85% as determined by SDS-PAGE
• Synonyms: TSHR; TSHR Protein; Human; Recombinant (ECD; His Tag); Human TSH Receptor/TSHR (His Tag); CHNG1 Protein; hTSHR-I Protein; LGR3 Protein; TSH Receptor Protein; TSHR recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 150mM NaCl, 10% Glycerol, 1mM TCEP, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly21-Gly413

• Source: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Gly
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human TSHR (NP_000360.2)(Gly21-Gly413) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: It is recommended that sterile water (1600 ul) be added to the vial to concentration is mesured by UV-Vis.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

(Human TSH Receptor/TSHR (His Tag))

Related Product Information for TSHR recombinant protein

Background: Thyroid-stimulating hormone (TSH) is secreted by the pituitary gland and promotes thyroid growth and function, with increased TSH levels typically associated with hypothyroidism. Immunohistochemical analysis revealed predominantly nuclei/peri-nuclei localization of TSHR in cancerous tissues but cell membrane localization in non-cancerous parts. Overexpression of TSHR was found in a great majority of HCC tissues and associated with unfavorable prognosis.

NCBI and Uniprot Product Information

• NCBI GI #: 64085121
• NCBI GeneID: 7253
• NCBI Accession #: NP_000360.2
• NCBI GenBank Nucleotide #: NM_000369.2
• UniProt Accession #: P16473; Q16503; Q8TB90; Q96GT6; Q9P1V4; Q9ULA3; Q9UPH3; A0PJU7; F5GYU5; G3V2A9
• Molecular Weight: The recombinant human TSHR consists of 404 amino acids and predicts a molecular mass of 4631 kDa.
• NCBI Official Full Name: thyrotropin receptor isoform 1
• NCBI Official Synonym Full Names: thyroid stimulating hormone receptor
• NCBI Official Symbol: TSHR
• NCBI Official Synonym Symbols: LGR3; CHNG1; hTSHR-I
• NCBI Protein Information: thyrotropin receptor; thyrotropin receptor-I, hTSHR-I; seven transmembrane helix receptor; thyroid stimulating hormone receptor, isoform 2
• UniProt Protein Name: Thyrotropin receptor
• Protein Family: Thyrotropin receptor
• UniProt Gene Name: TSHR
• UniProt Synonym Gene Names: LGR3; TSH-R
• UniProt Entry Name: TSHR_HUMAN

NCBI Description

The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

Uniprot Description

TSHR: Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5). Defects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH- independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers). Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation. Defects in TSHR are the cause of congenital hypothyroidism non-goitrous type 1 (CHNG1); also known as congenital hypothyroidism due to TSH resistance. CHNG1 is a non-autoimmune condition characterized by resistance to thyroid- stimulating hormone (TSH) leading to increased levels of plasma TSH and low levels of thyroid hormone. CHNG1 presents variable severity depending on the completeness of the defect. Most patients are euthyroid and asymptomatic, with a normal sized thyroid gland. Only a subset of patients develop hypothyroidism and present a hypoplastic thyroid gland. Defects in TSHR are the cause of familial gestational hyperthyroidism (HTFG). HTFG is a condition characterized by abnormally high levels of serum thyroid hormones occurring during early pregnancy. Defects in TSHR are the cause of hyperthyroidism non- autoimmune (HTNA). It is a condition characterized by abnormally high levels of serum thyroid hormones, thyroid hyperplasia, goiter and lack of anti-thyroid antibodies. Typical features of Graves disease such as exophthalmia, myxedema, antibodies anti-TSH receptor and lymphocytic infiltration of the thyroid gland are absent. Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 14q31

Cellular Component: integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: protein binding; thyroid-stimulating hormone receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; cell-cell signaling; positive regulation of cell proliferation

Disease: Hyperthyroidism, Nonautoimmune; Hypothyroidism, Congenital, Nongoitrous, 1; Hyperthyroidism, Familial Gestational