Exosome Component 9/EXOSC9 Recombinant Protein | EXOSC9 recombinant protein

Exosome Component 9/EXOSC9 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8122079

• Gene Names: EXOSC9; p5; p6; RRP45; PMSCL1; Rrp45p; PM/Scl-75
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Exosome Component 9/EXOSC9; Exosome Component 9/EXOSC9 Protein; Human; Recombinant (His Tag); Human EXOSC9 Protein (His Tag); p5 Protein; p6 Protein; PM/Scl-75 Protein; PMSCL1 Protein; RRP45 Protein; Rrp45p Protein; exosome component 9; EXOSC9 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM PB, pH7.0, 150mM NaCl, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn439

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human EXOSC9 (NP_005024.2)(Met1-Asn439) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 77812670
• NCBI GeneID: 5393
• NCBI Accession #: NP_005024.2
• NCBI GenBank Nucleotide #: NM_005033.2
• UniProt Accession #: Q06265; Q12883; Q4W5P5; Q86Y41; Q86Y48
• Molecular Weight: 41,089 Da
• NCBI Official Full Name: exosome complex component RRP45 isoform 2
• NCBI Official Synonym Full Names: exosome component 9
• NCBI Official Symbol: EXOSC9
• NCBI Official Synonym Symbols: p5; p6; RRP45; PMSCL1; Rrp45p; PM/Scl-75
• NCBI Protein Information: exosome complex component RRP45
• UniProt Protein Name: Exosome complex component RRP45
• Protein Family: Exosome complex component
• UniProt Gene Name: EXOSC9
• UniProt Synonym Gene Names: PMSCL1; PM/Scl-75
• UniProt Entry Name: EXOS9_HUMAN

NCBI Description

This gene encodes a component of the human exosome, a exoribonuclease complex which processes and degrades RNA in the nucleus and cytoplasm. This component may play a role in mRNA degradation and the polymyositis/scleroderma autoantigen complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

EXOSC9: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC9 binds to ARE-containing RNAs. Belongs to the RNase PH family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.13.-; Ribonuclease; RNA processing; Nucleolus

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: nucleoplasm; nuclear chromosome; intermediate filament cytoskeleton; cytoplasm; nucleolus; exosome (RNase complex); nucleus; cytosol; nuclear exosome (RNase complex)

Molecular Function: protein binding; 3'-5'-exoribonuclease activity; exoribonuclease activity; RNA binding; AU-rich element binding

Biological Process: immune response; gene expression; positive regulation of cell growth; rRNA processing; mRNA catabolic process, deadenylation-dependent decay