EIF5 recombinant protein

EIF5 Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121592

• Gene Names: EIF5; EIF-5; EIF-5A
• Purity: >95% as determined by SDS-PAGE
• Synonyms: EIF5; EIF5 Protein; Human; Recombinant (GST Tag); Human EIF-5A/EIF5 Protein (GST Tag); EIF-5 Protein; EIF-5A Protein; eukaryotic translation initiation factor 5; EIF5 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp150

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the mature form of human EIF5 (P55010)(Met1-Asp150) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for EIF5 recombinant protein

Background: EIF-5A, also known as EIF5, functions in start site selection as a GTPase accelerating protein (GAP) for the eukaryotic translation initiation factor (eIF) 2-GTP-tRNA ternary complex within the ribosome-bound pre-initiation complex. In protein synthesis initiation, eIF2 functions in its GTP-bound state to deliver initiator methionyl-tRNA to the small ribosomal subunit and is necessary for protein synthesis in all cells. EIF-5A stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). EIF-5A also interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit.

Product Categories/Family for EIF5 recombinant protein

Actin Dynamics Signaling Pathway

References

Si K, et al. (1996) Charactatierizon of multiple mRNAs that encode mammalian translation initiation factor 5 (eIF-5). J Biol Chem. 71(28):16934.Das S, et al. (1998) Specific interaction of eukaryotic translation initiation factor 5 (eIF5) with the beta-subunit of eIF2. J Biol Chem. 272(50):31712-8.Conte MR, et al. (2006) Structure of the eukaryotic initiation factor (eIF) 5 reveals a fold common to several translation factors. Biochemistry. 45(14):4550-8.

NCBI and Uniprot Product Information

• NCBI GI #: 21361337
• NCBI GeneID: 1983
• NCBI Accession #: NP_001960
• NCBI GenBank Nucleotide #: NM_001969
• UniProt Accession #: P55010; Q53XB3; Q9H5N2; Q9UG48
• Molecular Weight: 49,223 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 5
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 5
• NCBI Official Symbol: EIF5
• NCBI Official Synonym Symbols: EIF-5; EIF-5A
• NCBI Protein Information: eukaryotic translation initiation factor 5
• UniProt Protein Name: Eukaryotic translation initiation factor 5
• UniProt Gene Name: EIF5
• UniProt Synonym Gene Names: eIF-5
• UniProt Entry Name: IF5_HUMAN