IDO2 recombinant protein

IDO2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121475

• Gene Names: IDO2; INDOL1
• Purity: >93% as determined by SDS-PAGE
• Synonyms: IDO2; IDO2 Protein; Human; Recombinant (His Tag); Human IDO2 Protein (His Tag); INDOL1 Protein; indoleamine 2; 3-dioxygenase 2; IDO2 recombinant protein

• Host: E Coli
• Purity/Purification: >93% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 20% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met14-Gly420

• Species: Human
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human IDO2 (Q6ZQW0-1)(Met 14-Gly 420) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for IDO2 recombinant protein

Background: IDO2 belongs to the indoleamine 2,3-dioxygenase family. Indoleamine 2,3-dioxgyenase (IDO), is a cytosolic haem protein which, together with the hepatic enzyme tryptophan 2,3-dioxygenase, catalyzes the conversion of tryptophan and other indole derivatives to kynurenines. In addition to classic IDO (IDO1), a new variant, IDO2, has recently been described. IDO2 is expressed in the liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. IDO is widely distributed in human tissues, its physiological role is not fully understood but is of great interest. IDO can be up-regulated via cytokines such as interferon-gamma, and can thereby modulate the levels of tryptophan, which is vital for cell growth. In humans and mice, the IDO1 and IDO2 genes are present tandemly in a tail-to-head arrangement on chromosome 8. In lower vertebrates such as zebrafish and toads, only a single IDO gene may be present that may be more IDO2-like in structure. This closer relationship to IDO2 suggests that IDO2 may be the ancestor of the better characterized IDO1 gene and that IDO1 might have been generated by gene duplication of IDO2 before the origin of tetrapods in mammalian evolutionary history. IDO2 catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism.

References

Witkiewicz AK, et al. (2009) Genotyping and expression analysis of IDO2 in human pancreatic cancer: a novel, active target. J Am Coll Surg. 208 (5): 781-7.Sorensen RB, et al. (2011) Spontaneous cytotoxic T-Cell reactivity against indoleamine 2,3-dioxygenase-2. Cancer Res. 71 (6): 2038-44.Witkiewicz AK, et al. (2009) Genotyping and expression analysis of IDO2 in human pancreatic cancer: a novel, active target. J Am Coll Surg. 208 (5): 781-7.

NCBI and Uniprot Product Information

• NCBI GI #: 215274147
• NCBI GeneID: 169355
• UniProt Accession #: Q6ZQW0; A4UD41
• Molecular Weight: 19,610 Da
• NCBI Official Full Name: Indoleamine 2,3-dioxygenase 2
• NCBI Official Synonym Full Names: indoleamine 2,3-dioxygenase 2
• NCBI Official Symbol: IDO2
• NCBI Official Synonym Symbols: INDOL1
• NCBI Protein Information: indoleamine 2,3-dioxygenase 2; IDO-2; indoleamine 2,3-dioxygenase-like 1 protein; indoleamine 2,3-dioxygenase-like protein 1; indoleamine-pyrrole 2,3 dioxygenase-like 1; indoleamine-pyrrole 2,3-dioxygenase-like protein 1
• UniProt Protein Name: Indoleamine 2,3-dioxygenase 2
• Protein Family: Indoleamine 2,3-dioxygenase
• UniProt Gene Name: IDO2
• UniProt Synonym Gene Names: INDOL1; IDO-2
• UniProt Entry Name: I23O2_HUMAN

NCBI Description

Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]).[supplied by OMIM, Feb 2011]

Uniprot Description

INDOL1: Catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. Belongs to the indoleamine 2,3-dioxygenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tryptophan; Oxidoreductase; EC 1.13.11.-

Chromosomal Location of Human Ortholog: 8p11.21

Cellular Component: cytoplasm; cytosol

Molecular Function: metal ion binding; heme binding; indoleamine 2,3-dioxygenase activity; tryptophan 2,3-dioxygenase activity

Biological Process: tryptophan catabolic process to kynurenine; tryptophan catabolic process