SDS-Page
DDOST recombinant protein
DDOST Protein, Human, Recombinant
Gene Names
DDOST; OST; WBP1; AGER1; CDG1R; OST48; OKSWcl45
Purity
>95% as determined by SDS-PAGE
Synonyms
DDOST; DDOST Protein; Human; Recombinant; Human DDOST/OST48 Protein; AGER1 Protein; CDG1R Protein; OKSWcl45 Protein; OST Protein; OST48 Protein; WBP1 Protein; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic); DDOST recombinant protein
Host
E Coli
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50mM Tris, 150mM NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Ser43-Pro427
Species
Human
Predicted N Terminal
Gly
Tag
N-cleavage
Protein Construction
A DNA sequence encoding the human DDOST (P39656-1) extracellular domain (Ser 43-Pro 427) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for DDOST recombinant protein
Background: The enzyme oligosaccharyltransferase (dolichyl-diphosphooligosaccharide-protein glycosyltransferase)(DDOST), or 48-kDa subunit (OST48) is one of the catalytic subunits in this complex, exerts a typical type I membrane topology, containing a large luminal domain, a hydrophobic transmembrane domain and a short cytosolic peptide tail. DDOST/OST48 catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. The mammalian oligosaccharyltransferase (OST) is an oligomeric complex composed of three type I transmembrane proteins of the endoplasmic reticulum: ribophorin I (RI), ribophorin II (RII), and OST48. OST48 is not a glycoprotein and is not recognized by antibodies to either ribophorin. Like ribophorins I and II, OST48 was found to be an integral membrane protein, with the majority of the polypeptide located within the lumen of the endoplasmic reticulum (ER). OST48 does not show significant amino acid sequence homology to either ribophorin I or II. It had been found that only the luminal domain of RI contains ER retention information. The dilysine motif in OST48 functions as an ER localization motif because OST48 in which the two lysine residues are replaced by serine (OST48ss) is no longer retained in the ER and is found instead also at the plasma membrane.
References
Silberstein S, et al. (1992) The 48-kDa subunit of the mammalian oligosaccharyltransferase complex is homologous to the essential yeast protein WBP1. J Biol Chem. 267(33): 23658-63.Fu J, et al. (1997) Interactions among subunits of the oligosaccharyltransferase complex. J Biol Chem. 272(47): 29687-92.Yamagata T, et al. (1997) Genomics. Genome organization of human 48-kDa oligosaccharyltransferase (DDOST). 45(3): 535-40.Fu J, et al. (2000) Retention of subunits of the oligosaccharyltransferase complex in the endoplasmic reticulum. J Biol Chem. 275(6): 3984-90.Hardt B, et al. (2001) Analysis of structural signals conferring localisation of pig OST48 to the endoplasmic reticulum. Biol Chem. 382(7): 1039-47.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,020 Da
NCBI Official Full Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
NCBI Official Synonym Full Names
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic)
NCBI Official Symbol
DDOST
NCBI Official Synonym Symbols
OST; WBP1; AGER1; CDG1R; OST48; OKSWcl45
NCBI Protein Information
dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; advanced glycation endproduct receptor 1; dolichyl-diphosphooligosaccharide-protein glycotransferase; oligosaccharyl transferase 48 kDa subunit; oligosaccharyltransferase 48 kD
UniProt Protein Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
UniProt Gene Name
DDOST
UniProt Synonym Gene Names
KIAA0115; OST48; DDOST 48 kDa subunit; Oligosaccharyl transferase 48 kDa subunit
UniProt Entry Name
OST48_HUMAN
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Product Notes
The DDOST ddost (Catalog #AAA8121173) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Ser43-Pro4 27. It is sometimes possible for the material contained within the vial of "DDOST, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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