LYPLA2 recombinant protein

LYPLA2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121155

• Gene Names: LYPLA2; APT2; APT-2; DJ886K2.4
• Purity: >96% as determined by SDS-PAGE
• Synonyms: LYPLA2; LYPLA2 Protein; Human; Recombinant (His Tag); Human LYPLA2/APT-2 Protein (His Tag); APT-2 Protein; APT2 Protein; DJ886K2.4 Protein; lysophospholipase II; LYPLA2 recombinant protein

• Host: E Coli
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 0.05% Bring, pH8.2. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val231

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human LYPLA2 (O95372)(Met 1-Val 231) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LYPLA2 recombinant protein

Background: Lysophospholipase II (LYPLA2, LPL-II, or LysoPLA II), also known as Acyl-protein thioesterase 2 (APT-2), belongs to the AB hydrolase 2 family. This enzyme has lysophospholipase activity and may hydrolyze fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acyl-protein thioesterase 1 (APT-1) and Acyl-protein thioesterase 2 (APT-2) are cytosolic lysophospholipids hydrolyzing enzymes. The serum activity of APT-1 may play an important role in the determination of the concentration of des-acyl ghrelin in circulation, especially under septic inflammation. APT-2/LYPLA2 is expressed both in CHO-K1 and HeLa cells and its overexpression increased the deacylation rate of single acylated GAP-43 and affected the steady-state localization of diacylated GAP-43 and H-Ras. Thus, the results demonstrate that APT-2/LYPLA2 is the protein thioesterase involved in the acylation/deacylation cycle operating in GAP-43 subcellular distribution.

References

Satou M, et al. (2010) Identification and characterization of acyl-protein thioesterase 1/lysophospholipase I as a ghrelin deacylation/lysophospholipid hydrolyzing enzyme in fetal bovine serum and conditioned medium. Endocrinology. 151(10): 4765-75.Tomatis VM, et al. (2010) Acyl-protein thioesterase 2 catalyzes the deacylation of peripheral membrane-associated GAP-43. PLoS One. 5(11): e15045.

NCBI and Uniprot Product Information

• NCBI GI #: 9966764
• NCBI GeneID: 11313
• NCBI Accession #: NP_009191.1
• NCBI GenBank Nucleotide #: NM_007260.2
• UniProt Accession #: O95372; Q7Z4Z2
• Molecular Weight: 24,737 Da
• NCBI Official Full Name: acyl-protein thioesterase 2
• NCBI Official Synonym Full Names: lysophospholipase II
• NCBI Official Symbol: LYPLA2
• NCBI Official Synonym Symbols: APT2; APT-2; DJ886K2.4
• NCBI Protein Information: acyl-protein thioesterase 2; LPL-II; lysoPLA II
• UniProt Protein Name: Acyl-protein thioesterase 2
• Protein Family: Acyl-protein thioesterase
• UniProt Gene Name: LYPLA2
• UniProt Synonym Gene Names: APT2; APT-2; LPL-II; LysoPLA II
• UniProt Entry Name: LYPA2_HUMAN

NCBI Description

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. There are alternatively spliced transcript variants described for this gene but the full length nature is not known yet. [provided by RefSeq, Jul 2008]

Uniprot Description

LYPLA2: May hydrolyze fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has lysophospholipase activity. Deacylates GAP43. Belongs to the AB hydrolase 2 family.

Protein type: EC 3.1.2.-; Phospholipase; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: cytoplasm

Molecular Function: hydrolase activity

Biological Process: fatty acid metabolic process