NRAS recombinant protein

NRAS Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121074

• Gene Names: NRAS; NS6; ALPS4; N-ras; NRAS1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: NRAS; NRAS Protein; Human; Recombinant (His Tag); Human NRAS Protein (His Tag); ALPS4 Protein; CMNS Protein; N-ras Protein; NCMS Protein; NRAS1 Protein; NS6 Protein; neuroblastoma RAS viral (v-ras) oncogene homolog; NRAS recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 0.1M NaCl, 10% Glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys186

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human NRAS (P01111)(Met1-Cys186) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NRAS recombinant protein

Background: NRAS was discovered by researchers at the Institute of Cancer Research, funded by the Cancer Research Campaign (now Cancer Research UK). NRAS gene is a member of the Ras gene family. It is mapped on chromosome 1, and it is activated in HL6, a promyelocytic leukemia line. The mammalian ras gene family consists of the Harvey and Kirsten ras genes (HRAS and KRAS), an inactive pseudogene of each (c-Hras2 and c-Kras1), and the N-ras gene. They differ significantly only in the C-terminal 4 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. The NRAS gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The NRAS gene consists of seven exons (-I, I, II, III, IV, V, VI).

Product Categories/Family for NRAS recombinant protein

Cancer Drug Targets; EGFR Signaling Pathway

References

Marshall CJ. et al., 1982, Nature. 299 (5879): 171-3. Hall Alan. et al., 1983, Nature. 303 (5916): 396-400. McCormick F. 1996, Mol Reprod Dev. 42 (4): 500-6.

NCBI and Uniprot Product Information

• NCBI GI #: 4505451
• NCBI GeneID: 4893
• NCBI Accession #: NP_002515.1
• NCBI GenBank Nucleotide #: NM_002524.4
• UniProt Accession #: P01111; Q14971; Q15104; Q15282
• Molecular Weight: 21kDa
• NCBI Official Full Name: GTPase NRas
• NCBI Official Synonym Full Names: neuroblastoma RAS viral (v-ras) oncogene homolog
• NCBI Official Symbol: NRAS
• NCBI Official Synonym Symbols: NS6; ALPS4; N-ras; NRAS1
• NCBI Protein Information: GTPase NRas; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog
• UniProt Protein Name: GTPase NRas
• Protein Family: GTPase
• UniProt Gene Name: NRAS
• UniProt Synonym Gene Names: HRAS1
• UniProt Entry Name: RASN_HUMAN