REG1A recombinant protein

REG1A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120754

• Gene Names: REG1A; P19; PSP; PTP; REG; ICRF; PSPS; PSPS1
• Purity: >97% as determined by SDS-PAGE
• Synonyms: REG1A; REG1A Protein; Human; Recombinant (His Tag); Human REG1A/PSPS Protein (His Tag); ICRF Protein; MGC12447 Protein; P19 Protein; PSP Protein; PSPS Protein; PSPS1 Protein; PTP Protein; R EG Protein; REG Protein; regenerating family member 1 alpha; REG1A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn166

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human REG1A (NP_002900.2)(Met 1-Asn 166) precursor was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for REG1A recombinant protein

Background: Regenerating (reg) gene encodes protein that has been involved in pancreatic lithogenesis and the regeneration of islet cells and therefore the abnormality of reg genes could be associated with fibrocalculous pancreatopathy. REG I has been shown to be crucial for induction of ductal epithelial cells to differentiate into some cells. Lithostathine-1-alpha, also known as Pancreatic stone protein, Pancreatic thread protein, Regenerating islet-derived protein 1-alpha, REG1A, REG-1-alpha, and PSPS, is highly expressed in fetal and infant brains. REG1A contains one C-type lectin domain and is a known growth factor affecting pancreatic islet beta cells. REG1A may act as an inhibitor of spontaneous calcium carbonate precipitation. It may also be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. REG1A has been reported to be expressed in human cancers, and it may be positively correlated with patient's prognosis. REG3A and REG1A proteins are both involved in liver and pancreatic regeneration and proliferation. High levels of REG1A expression by tumor cells are an independent predictor of a poor prognosis in patients with non-small cell lung cancer (NSCLC).

References

Boonyasrisawat W, et al. (2002) Analysis of the reg1alpha and reg1beta gene transcripts in patients with fibrocalculous pancreatopathy. Southeast Asian J Trop Med Public Health. 33(2): 365-72.Tezel E, et al. (2004) REG I as a marker for human pancreatic acinoductular cells. Hepatogastroenterology. 51(55): 91-6.Geng J, et al. (2009) REG1A predicts recurrence in stage Ta/T1 bladder cancer. Eur J Surg Oncol. 35(8): 852-7.

NCBI and Uniprot Product Information

• NCBI GI #: 29725633
• NCBI GeneID: 5967
• NCBI Accession #: NP_002900.2
• NCBI GenBank Nucleotide #: NM_002909.4
• UniProt Accession #: P05451; P11379; Q4ZG28
• Molecular Weight: 18,731 Da
• NCBI Official Full Name: lithostathine-1-alpha
• NCBI Official Synonym Full Names: regenerating islet-derived 1 alpha
• NCBI Official Symbol: REG1A
• NCBI Official Synonym Symbols: P19; PSP; PTP; REG; ICRF; PSPS; PSPS1
• NCBI Protein Information: lithostathine-1-alpha; REG-1-alpha; islet cells regeneration factor; islet of langerhans regenerating protein; lithostathine 1 alpha; pancreatic stone protein, secretory; pancreatic thread protein; protein-X; regenerating islet-derived protein 1-alpha; re
• UniProt Protein Name: Lithostathine-1-alpha
• Protein Family: Lithostathine
• UniProt Gene Name: REG1A
• UniProt Synonym Gene Names: PSPS; PSPS1; REG; ICRF; REG; PSP; PTP; REG-1-alpha
• UniProt Entry Name: REG1A_HUMAN

NCBI Description

This gene is a type I subclass member of the Reg gene family. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication. [provided by RefSeq, Jul 2008]

Uniprot Description

REG1A: Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p12

Molecular Function: growth factor activity; carbohydrate binding

Biological Process: positive regulation of cell proliferation