GADD45A recombinant protein

GADD45A Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8120705

• Gene Names: GADD45A; DDIT1; GADD45
• Purity: >95% as determined by SDS-PAGE
• Synonyms: GADD45A; GADD45A Protein; Human; Recombinant (His & GST Tag); Human GADD45A/DDIT-1 Protein (His & GST Tag); DDIT1 Protein; GADD45 Protein; growth arrest and DNA-damage-inducible; alpha; GADD45A recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg165

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the full length of human GADD45A (P24522)(Met 1-Arg 165) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GADD45A recombinant protein

Background: GADD45A is a member of the GADD45 Family, and has been found to associate with several cytoplasmic and nuclear factors and has been implicated in several cellular functions, including MAPK signaling, cell cycle regulation, DNA repair and genomic stability, apoptosis, and immune responses. The GADD45 Family of genes is rapidly induced by different stressors, including differentiation-inducing cytokines, and there is a large body of evidence that their cognate proteins are key players in cellular stress responses. GADD45A protein has been reported to interact with multiple important cellular proteins, including Cdc2 protein kinase, proliferating cell nuclear antigen (PCNA), p21Waf1/Cip1 protein, core histone protein and MTK/MEKK4, an up-stream activator of the JNK/SAPK pathway, indicating that GADD45A may play important roles in the control of cell cycle checkpoint, DNA repair process, and signaling transduction. GADD45A expression in response to genotoxic stress illustrates a more complex scenario, wherein transcriptional changes operate in concert with mRNA turnover and translational regulation. GADD45A was the first stress-inducible gene determined to be up-regulated by p53 and is also a target for the p53 homologues, p63 and p73. The decreased GADD45A expression is also considered a survival mechanism, as cancer cells without this control can evade the apoptotic pathway leading to increased tumourigenesis. As GADD45A is an essential component of many metabolic pathways that control proliferating cancer cells, it presents itself as an emerging drug target worthy of further investigation.

Product Categories/Family for GADD45A recombinant protein

p53 Pathway

References

Zhan Q. (2005) Gadd45a, a p53- and BRCA1-regulated stress protein, in cellular response to DNA damage. Mutat Res. 569(1-2): 133-43.Lal A, et al. (2006) Egad, more forms of gene regulation: the gadd45a story. Cell Cycle. 5(13): 1422-5.Hoffman B, et al. (2007) Role of gadd45 in myeloid cells in response to hematopoietic stress. Blood Cells Mol Dis. 39(3): 344-7.Rosemary Siafakas A, et al. (2009) Growth arrest and DNA damage-45 alpha (GADD45alpha). Int J Biochem Cell Biol. 41(5): 986-9.

NCBI and Uniprot Product Information

• NCBI GI #: 315075325
• NCBI GeneID: 1647
• NCBI Accession #: NP_001186671.1
• NCBI GenBank Nucleotide #: NM_001199742.1
• UniProt Accession #: P24522; Q5TCA7; Q5TCA8
• Molecular Weight: 18,336 Da
• NCBI Official Full Name: growth arrest and DNA damage-inducible protein GADD45 alpha isoform 3
• NCBI Official Synonym Full Names: growth arrest and DNA-damage-inducible, alpha
• NCBI Official Symbol: GADD45A
• NCBI Official Synonym Symbols: DDIT1; GADD45
• NCBI Protein Information: growth arrest and DNA damage-inducible protein GADD45 alpha; DDIT-1; DNA damage-inducible transcript-1; DNA-damage-inducible transcript 1; DNA damage-inducible transcript 1 protein; growth arrest and DNA-damage-inducible 45 alpha
• UniProt Protein Name: Growth arrest and DNA damage-inducible protein GADD45 alpha
• Protein Family: Growth arrest and DNA damage-inducible protein
• UniProt Gene Name: GADD45A
• UniProt Synonym Gene Names: DDIT1; GADD45; DDIT-1
• UniProt Entry Name: GA45A_HUMAN

NCBI Description

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]

Uniprot Description

GADD45A: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Interacts with MAPK14. Interacts with GADD45GIP1. Interacts with PCNA. By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediate by some kinase(s) other than PKC. Belongs to the GADD45 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; DNA repair, damage; Nuclear receptor co-regulator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p31.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of apoptosis; apoptosis; centrosome cycle; activation of MAPKKK activity; DNA damage response, signal transduction; negative regulation of protein kinase activity; positive regulation of JNK cascade; regulation of cyclin-dependent protein kinase activity; DNA repair; cell cycle arrest