GPR114 Recombinant Protein | ADGRG5 recombinant protein

GPR114 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120525

• Gene Names: GPR114; PGR27
• Purity: >94% as determined by SDS-PAGE
• Synonyms: GPR114; GPR114 Protein; Human; Recombinant (hFc Tag); Human GPR114 Protein (Fc Tag); PGR27 Protein; ADGRG5 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly184

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 22
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human GPR114 (Q8IZF4)(Met1-Gly184) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for ADGRG5 recombinant protein

Background: GPR114 belongs to the G-protein coupled receptor 2 family. Members of this family share a common molecular architecture which consists of seven transmembrane domains, three extracellular loops, three intracellular loops, an amino-terminal extracellular domain, and an intracellular carboxyl terminus. It is thought that light acts as the activating stimulus of a G-protein-coupled receptor (GPCR). GPCRs are expected to have a molecular function (G-protein coupled receptor activity) and to localize in various compartments (endoplasmic reticulum membrane, plasma membrane, integral to the membrane). Family B of the GPCRs is a small but structurally and functionally diverse group of proteins that includes receptors for polypeptide hormones, molecules thought to mediate intercellular interactions at the plasma membrane, and a group of Drosophila proteins that regulate stress responses and longevity. GPR114 contains 1 GPS domain. GPR114 gene has been proposed to participate in processes (G-protein coupled receptor protein signaling pathway, neuropeptide signaling pathway).

References

Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36(1):40-5.Bjarnadttir TK, et al. (2005) The human and mouse repertoire of the adhesion family of G-protein-coupled receptors. Genomics. 84(1):23-33.Gerhard DS, et al. (2004) The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC). Genome Res. 14(10B):2121-7.

NCBI and Uniprot Product Information

• NCBI GI #: 24475871
• NCBI GeneID: 221188
• NCBI Accession #: NP_722579.1
• NCBI GenBank Nucleotide #: NM_153837.1
• UniProt Accession #: Q8IZF4; Q6ZMH7; Q6ZML4; Q86SL8; Q8IZ14; B3KXZ5
• Molecular Weight: 59,000 Da
• NCBI Official Full Name: probable G-protein coupled receptor 114
• NCBI Official Synonym Full Names: G protein-coupled receptor 114
• NCBI Official Symbol: GPR114
• NCBI Official Synonym Symbols: PGR27
• NCBI Protein Information: probable G-protein coupled receptor 114; G protein-coupled receptor PGR27; G-protein coupled receptor PGR27
• UniProt Protein Name: Probable G-protein coupled receptor 114
• Protein Family: Adhesion G protein-coupled receptor
• UniProt Gene Name: GPR114
• UniProt Synonym Gene Names: PGR27
• UniProt Entry Name: GP114_HUMAN

Uniprot Description

GPR114: Orphan receptor. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 2

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway