GM-CSF Receptor alpha Active Protein | CSF2RA active protein

GM-CSF Receptor alpha Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120439

• Gene Names: CSF2RA; GMR; CD116; CSF2R; SMDP4; CDw116; CSF2RX; CSF2RY; GMCSFR; CSF2RAX; CSF2RAY; GM-CSF-R-alpha
• Purity: >90% as determined by SDS-PAGE
• Synonyms: GM-CSF Receptor alpha; GM-CSF Receptor alpha Protein; Human; Recombinant (hFc Tag); Human CSF2RA/GM-CSFR/CD116 Protein (Fc Tag); CD116 Protein; CDw116 Protein; CSF2R Protein; CSF2RAX Protein; CSF2RAY Protein; CSF2RX Protein; CSF2RY Protein; GM-CSF-R-alpha Protein; GMCSFR Protein; GMR Protein; SMDP4 Protein; colony stimulating factor 2 receptor; alpha; low-affinity (granulocyte-macrophage); CSF2RA active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly320

• Species: Human
• Activity: Measured by its ability to inhibit GM-CSF dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 10-15ug/ml.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 23
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Gly 320) of human GM-CSFRalpha (NP_006131.2) pro-protein was expressed with the C-terminal fused Fc region of human IgG1.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for CSF2RA active protein

Background: CD116/GM-CSFR has been preferentially associated with M4, M5 subtype of AML but is not specific. The cluster of differentiation (cluster of designation)(often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules present on white blood cells initially but found in almost any kind of cell of the body, providing targets for immunophenotyping of cells. Physiologically, CD molecules can act in numerous ways, often acting as receptors or ligands (the molecule that activates a receptor) important to the cell. A signal cascade is usually initiated, altering the behavior of the cell (see cell signaling). Some CD proteins do not play a role in cell signaling, but have other functions, such as cell adhesion. CD116/GM-CSFR is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. CD116/GM-CSFR is found in the pseudoautosomal region (PAR) of the X and Y chromosomes.

References

Sjoblom C, et al. (2002) Granulocyte-macrophage colony-stimulating factor (GM-CSF) acts independently of the beta common subunit of the GM-CSF receptor to prevent inner cell mass apoptosis in human embryos. Biol Reprod. 67(6): 1817-23.Goldstein JI, et al. (2011) Defective leukocyte GM-CSF receptor (CD116) expression and function in inflammatory bowel disease. Gastroenterology. 141(1): 208-16.Saulle E, et al. (2009) Colocalization of the VEGF-R2 and the common IL-3/GM-CSF receptor beta chain to lipid rafts leads to enhanced p38 activation. Br J Haematol. 145(3): 399-411.

NCBI and Uniprot Product Information

• NCBI GI #: 20070195
• NCBI GeneID: 1438
• NCBI Accession #: NP_006131.2
• NCBI GenBank Nucleotide #: NM_006140.4
• UniProt Accession #: P15509; O00207; Q14429; Q14430; Q14431; Q16564; A7J003; A8KAM1; B4DW68; J3JS76; J3JS77
• Molecular Weight: 31,101 Da
• NCBI Official Full Name: granulocyte-macrophage colony-stimulating factor receptor subunit alpha isoform a
• NCBI Official Synonym Full Names: colony stimulating factor 2 receptor alpha subunit
• NCBI Official Symbol: CSF2RA
• NCBI Official Synonym Symbols: GMR; CD116; CSF2R; SMDP4; CDw116; CSF2RX; CSF2RY; GMCSFR; CSF2RAX; CSF2RAY; GM-CSF-R-alpha
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor receptor subunit alpha
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor receptor subunit alpha
• Protein Family: Granulocyte-macrophage colony-stimulating factor receptor
• UniProt Gene Name: CSF2RA
• UniProt Synonym Gene Names: CSF2R; CSF2RY; GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha
• UniProt Entry Name: CSF2R_HUMAN

NCBI Description

The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble. [provided by RefSeq, Jul 2008]

Uniprot Description

CSF2RA: Low affinity receptor for granulocyte-macrophage colony- stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. Defects in CSF2RA are the cause of pulmonary surfactant metabolism dysfunction type 4 (SMDP4). A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Belongs to the type I cytokine receptor family. Type 5 subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; receptor activity

Biological Process: response to ethanol; cytokine and chemokine mediated signaling pathway

Disease: Surfactant Metabolism Dysfunction, Pulmonary, 4