PDGFRB recombinant protein

PDGFRB Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA8120326

• Gene Names: PDGFRB; IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: PDGFRB; PDGFRB Protein; Human; Recombinant (ECD; hFc Tag); Human PDGFRB Protein (Fc Tag); CD140B Protein; IBGC4 Protein; IMF1 Protein; JTK12 Protein; KOGS Protein; PDGFR Protein; PDGFR-1 Protein; PDGFR1 Protein; PENTT Protein; platelet-derived growth factor receptor; beta polypeptide; PDGFRB recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Phe530

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Leu 33
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human PDGFRB (NP_002600.1)(Met1-Phe530) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PDGFRB recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-beta was correlated with depth of cancer invasion at statistically significant level.

Product Categories/Family for PDGFRB recombinant protein

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
Suzuki S, et al. (2010) Clinicopathological significance of platelet-derived growth factor (PDGF)-B and vascular endothelial growth factor-A expression, PDGF receptor- phosphorylation, and microvessel density in gastric cancer. BMC cancer. 10: 659.

NCBI and Uniprot Product Information

• NCBI GI #: 4505683
• NCBI GeneID: 5159
• NCBI Accession #: NP_002600.1
• NCBI GenBank Nucleotide #: NM_002609.3
• UniProt Accession #: P09619; Q8N5L4
• Molecular Weight: 123,968 Da
• NCBI Official Full Name: platelet-derived growth factor receptor beta
• NCBI Official Synonym Full Names: platelet-derived growth factor receptor, beta polypeptide
• NCBI Official Symbol: PDGFRB
• NCBI Official Synonym Symbols: IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1
• NCBI Protein Information: platelet-derived growth factor receptor beta; PDGFR-beta; PDGF-R-beta; CD140 antigen-like family member B; platelet-derived growth factor receptor 1; beta-type platelet-derived growth factor receptor
• UniProt Protein Name: Platelet-derived growth factor receptor beta
• Protein Family: Platelet-derived growth factor receptor
• UniProt Gene Name: PDGFRB
• UniProt Synonym Gene Names: PDGFR; PDGFR1; PDGF-R-beta; PDGFR-beta; PDGFR-1
• UniProt Entry Name: PGFRB_HUMAN

NCBI Description

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Jul 2008]

Uniprot Description

PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent.

Protein type: Kinase, protein; Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; Oncoprotein; TK group; PDGFR family

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: lysosomal lumen; focal adhesion; cell surface; membrane; apical plasma membrane; cytoplasm; cytoplasmic membrane-bound vesicle; integral to membrane; plasma membrane; nucleus; intrinsic to plasma membrane

Molecular Function: protein binding; platelet activating factor receptor activity; protein-tyrosine kinase activity; platelet-derived growth factor binding; platelet-derived growth factor beta-receptor activity; platelet-derived growth factor receptor binding; phosphoinositide 3-kinase binding; platelet-derived growth factor receptor activity; protein kinase binding; ATP binding; receptor binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; peptidyl-tyrosine phosphorylation; wound healing; nerve growth factor receptor signaling pathway; response to toxin; protein amino acid autophosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; platelet-derived growth factor receptor signaling pathway; cardiac myofibril assembly; signal transduction; positive regulation of smooth muscle cell migration; response to estradiol stimulus; smooth muscle development; positive regulation of MAP kinase activity; positive regulation of cell proliferation; tissue homeostasis; phosphatidylinositol metabolic process; regulation of peptidyl-tyrosine phosphorylation; inner ear development; epidermal growth factor receptor signaling pathway; cell migration; fibroblast growth factor receptor signaling pathway; response to retinoic acid; phosphoinositide-mediated signaling; positive regulation of mitosis; in utero embryonic development; positive regulation of chemotaxis; adrenal gland development; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; glycosaminoglycan biosynthetic process; G-protein coupled receptor protein signaling pathway; response to hyperoxia; response to hydrogen peroxide; skeletal morphogenesis; regulation of actin cytoskeleton organization and biogenesis; innate immune response; negative regulation of apoptosis; positive regulation of cell migration

Disease: Myeloproliferative Disorder, Chronic, With Eosinophilia; Myofibromatosis, Infantile, 1; Basal Ganglia Calcification, Idiopathic, 1; Basal Ganglia Calcification, Idiopathic, 4