DcR3 Active Protein | TNFRSF6B active protein

DcR3 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120116

• Gene Names: TNFRSF6B; M68; TR6; DCR3; M68E; DJ583P15.1.1
• Purity: >85% as determined by SDS-PAGE
• Synonyms: DcR3; DcR3 Protein; Human; Recombinant (hFc Tag); Human DCR3/TNFRSF6B Protein (Fc Tag); DCR3 Protein; DJ583P15.1.1 Protein; M68 Protein; M68E Protein; TNFRSF6B Protein; TR6 Protein; tumor necrosis factor receptor superfamily member 6b; TNFRSF6B active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM Glycine, 10mM NaCl, pH7.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His300

• Species: Human
• Activity: Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.5-3ug/mL in the presence of 200ng/mL recombinant human Fas ligand.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Val 30
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human DCR3 (O95407)(Met1-His300) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.5-3 ug/mL in the presence of 200 ng/mL recombinant human Fas ligand.)

Related Product Information for TNFRSF6B active protein

Background: Tumor necrosis factor receptor superfamily member 6B (TNFRSF6B) also known as DcR3(Decoy Receptor 3) and M68 is the tumor necrosis factor receptor superfamily. DcR3/TNFRSF6B belongs to the tumor necrosis factor receptor superfamily. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death. It acts as a decoy receptor that competes with death receptors for ligand binding. Over-expression of this gene has been noted in gastrointestinal tract tumors. Read-through transcription into this gene from the neighboring upstream gene, which encodes regulator of telomere elongation helicase 1 (RTEL1), generates a non-coding transcript. DcR3/TNFRSF6B is detected in fetal lung, brain and liver. DcR3/TNFRSF6B is also detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. This protein is highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.

Product Categories/Family for TNFRSF6B active protein

Death Receptor Signaling

References

Migone TS, et al. (2002) TL1A is a TNF-like ligand for DR3 and TR6/DcR3 and functions as a T cell costimulator. Immunity. 16(3): 479-92.Takahama Y, et al. (2002) The prognostic significance of overexpression of the decoy receptor for Fas ligand (DcR3) in patients with gastric carcinomas. Gastric Cancer. 5(2): 61-8.Zhang J, et al. (2001) Modulation of T-cell responses to alloantigens by TR6/DcR3. J Clin Invest. 107(11): 1459-68.

NCBI and Uniprot Product Information

• NCBI GI #: 4507585
• NCBI GeneID: 8771
• NCBI Accession #: NP_003814.1
• NCBI GenBank Nucleotide #: NM_003823.3
• UniProt Accession #: O95407
• Molecular Weight: 32,680 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 6B
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 6b, decoy
• NCBI Official Symbol: TNFRSF6B
• NCBI Official Synonym Symbols: M68; TR6; DCR3; M68E; DJ583P15.1.1
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6B; decoy receptor 3; decoy receptor for Fas ligand
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6B
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF6B
• UniProt Synonym Gene Names: DCR3; TR6; DcR3
• UniProt Entry Name: TNF6B_HUMAN

NCBI Description

This gene belongs to the tumor necrosis factor receptor superfamily. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death. It acts as a decoy receptor that competes with death receptors for ligand binding. Over-expression of this gene has been noted in gastrointestinal tract tumors. Read-through transcription into this gene from the neighboring upstream gene, which encodes regulator of telomere elongation helicase 1 (RTEL1), generates a non-coding transcript. [provided by RefSeq, Feb 2011]

Uniprot Description

TNFRSF6B: Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis. Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: extracellular space

Molecular Function: protein binding; receptor activity

Biological Process: apoptosis; negative regulation of apoptosis