IGFBP5 active protein

IGFBP5 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120099

• Gene Names: IGFBP5; IBP5
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IGFBP5; IGFBP5 Protein; Human; Recombinant (His Tag); Human IGFBP5/IGFBP-5 Protein (His Tag); IBP5 Protein; insulin-like growth factor binding protein 5; IGFBP5 active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, pH8.0, 300mM NaCl, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu272

• Species: Human
• Activity: Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. The ED50 for this effect is 0.1-0.5ug/mL in the presence of 14ng/mL rhIGF-II.
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Leu 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human IGFBP5 (NP_000590.1)(Met1-Glu272) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. The ED50 for this effect is 0.1-0.5 ug/mL in the presence of 14 ng/mL rhIGF-II.)

NCBI and Uniprot Product Information

• NCBI GI #: 10834982
• NCBI GeneID: 3488
• NCBI Accession #: NP_000590.1
• NCBI GenBank Nucleotide #: NM_000599.3
• UniProt Accession #: P24593; Q5U0A3
• Molecular Weight: 30,570 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 5
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 5
• NCBI Official Symbol: IGFBP5
• NCBI Official Synonym Symbols: IBP5
• NCBI Protein Information: insulin-like growth factor-binding protein 5; IBP-5; IGFBP-5; IGF-binding protein 5
• UniProt Protein Name: Insulin-like growth factor-binding protein 5
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP5
• UniProt Synonym Gene Names: IBP5; IBP-5; IGF-binding protein 5; IGFBP-5
• UniProt Entry Name: IBP5_HUMAN

Uniprot Description

IGFBP5: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Cell cycle regulation; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region

Molecular Function: protein binding; insulin-like growth factor I binding; fibronectin binding; insulin-like growth factor II binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; mammary gland involution; hair follicle morphogenesis; negative regulation of smooth muscle cell proliferation; negative regulation of insulin-like growth factor receptor signaling pathway; glucose metabolic process; negative regulation of smooth muscle cell migration; signal transduction; glucose homeostasis; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; negative regulation of translation; striated muscle cell differentiation; negative regulation of osteoblast differentiation; regulation of cell growth; skeletal muscle growth; negative regulation of cell migration