Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse Interferon gamma expression on BABL/c splenocytes. BABL/c splenocytes were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer's manual, stained with FITC-conjugated anti-Mouse Interferon gamma and APC-conjugated anti-Mouse CD4. The dot plots were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Mouse Interferon Gamma Monoclonal Antibody | anti-IFNG antibody
Recombinant Anti-Interferon Gamma Antibody (FITC), Rabbit Monoclonal
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
Interferon Gamma; Monoclonal Antibody; Recombinant Anti-Interferon Gamma Antibody (FITC); Rabbit Monoclonal; IFNG/Interferon Gamma Antibody (FITC); Rabbit MAb; Anti-Ifg Antibody; Anti-IFN-g Antibody; interferon; gamma; anti-IFNG antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
373
Specificity
Mouse IFNG/Interferon Gamma
Purity/Purification
Protein A
Form/Format
Liquid. Aqueous solution containing 0.5% BSA and 0.03% ProClin300
Concentration
5ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-IFNG antibody
Flow Cytometry (FC/FACS)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse IFNG/Interferon Gamma Protein
Conjugation
FITC
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IFNG/Interferon Gamma (rM IFNG/Interferon Gamma; NP_032363.1; Met1-Cys155) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze!
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse Interferon gamma expression on BABL/c splenocytes. BABL/c splenocytes were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer's manual, stained with FITC-conjugated anti-Mouse Interferon gamma and APC-conjugated anti-Mouse CD4. The dot plots were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse Interferon gamma expression on BABL/c splenocytes. BABL/c splenocytes were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer's manual, stained with FITC-conjugated anti-Mouse Interferon gamma and APC-conjugated anti-Mouse CD4. The dot plots were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-IFNG antibody
Background: IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six alpha-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and tumor control. Aberrant IFN gamma expression is associated with some autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.
Product Categories/Family for anti-IFNG antibody
References
Gray P W, et al. (1982) Structure of the human immune interferon gene. Nature. 298: 859-63.
Taya Y, et al. (1982) Cloning and structure of the human immune interferon-gamma chromosomal gene. EMBO J. 1: 953-8.
Goshima N, et al. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nomura N Nat Methods. 5: 1011-7.
Thiel DJ, et al. (2000) Observation of an unexpected third receptor molecule in the crystal structure of human interferon-gamma receptor complex. Structure. 8 (9): 927-36.
Naylor SL, et al. (1983) Human immune interferon gene is located on chromosome 12. J Exp Med. 157 (3): 1020-7.
Schoenborn JR, et al. (2007) Regulation of interferon-gamma during innate and adaptive immune responses. Adv Immunol. 96: 41-101.
Taya Y, et al. (1982) Cloning and structure of the human immune interferon-gamma chromosomal gene. EMBO J. 1: 953-8.
Goshima N, et al. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nomura N Nat Methods. 5: 1011-7.
Thiel DJ, et al. (2000) Observation of an unexpected third receptor molecule in the crystal structure of human interferon-gamma receptor complex. Structure. 8 (9): 927-36.
Naylor SL, et al. (1983) Human immune interferon gene is located on chromosome 12. J Exp Med. 157 (3): 1020-7.
Schoenborn JR, et al. (2007) Regulation of interferon-gamma during innate and adaptive immune responses. Adv Immunol. 96: 41-101.
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Product Notes
The IFNG (Catalog #AAA8119586) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Interferon Gamma Antibody (FITC), Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Interferon Gamma can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the IFNG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interferon Gamma, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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