RANKL Recombinant Protein | TNFSF11 recombinant protein

RANKL Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8118840

• Purity: >87 % as determined by SDS-PAGE
• Synonyms: RANKL; RANKL Protein; Mouse; Recombinant (hFc Tag); Mouse RANKL/OPGL/TNFSF11/CD254 Protein (Fc Tag); tumor necrosis factor (ligand) superfamily; member 11; Ly109l Protein; ODF Protein; OPG Protein; OPGL Protein; Trance Protein; TNFSF11 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >87 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 505aa
• Sequence: Arg72-Asp316

• Species: Mouse
• Tag: hFc
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu
• Protein Construction: |A DNA sequence encoding the extracellular domain of mouse TNFSF11 (AAC40113.1) (Arg 72-Asp 316) was fused with the Fc region of human IgG1 at the N-terminus
• Activity: Immobilized mouse Fc-TNFSF11 at 10ug/ml (100ul/well) can bind biotinylated human TNFRSF11B-His (Cat:10271-H08H). The EC50 of biotinylated human TNFRSF11B-His (Cat:10271-H08H) is 0.07-0.17ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Immobilized mouse Fc-TNFSF11 at 10 ug/ml (100 ul/well) can bind biotinylated human TNFRSF11B-His (Cat: 10271-H08H), The EC50 of biotinylated human TNFRSF11B-His (Cat: 10271-H08H) is 0.07-0.17 ug/ml.)

Related Product Information for TNFSF11 recombinant protein

Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type II membrane protein that belongs to the tumor necrosis factor family. The receptor activator of nuclear factor-kappaB ligand (RANKL), its cognate receptor RANK, and its natural decoy receptor osteoprotegerin have been identified as the final effector molecules of osteoclastic bone resorption. RANK and RANKL are key regulators of bone remodeling and regulate T cell/dendritic cell communications, and lymph node formation. Moreover, RANKL and RANK are expressed in mammary gland epithelial cells and control the development of a lactating mammary gland during pregnancy. Genetically, RANKL and RANK are essential for the development and activation of osteoclasts and bone loss in response to virtually all triggers tested. Inhibition of RANKL function via the natural decoy receptor osteoprotegerin (OPG, TNFRSF11B) prevents bone loss in postmenopausal osteoporosis and cancer metastases. Importantly, RANKL appears to be the pathogenetic principle that causes bone and cartilage destruction in arthritis. RANK-RANKL signaling not only activates a variety of downstream signaling pathways required for osteoclast development, but crosstalk with other signaling pathways also fine-tunes bone homeostasis both in normal physiology and disease. In addition, RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy.

Product Categories/Family for TNFSF11 recombinant protein

Cancer Drug Targets

References

Takayanagi H, et al. (2002) Signaling crosstalk between RANKL and interferons in osteoclast differentiation. Arthritis Res. 4 Suppl 3: S227-32.
Nakashima T, et al. (2003) RANKL and RANK as novel therapeutic targets for arthritis. Curr Opin Rheumatol. 15(3): 280-7.
Schwarz EM, et al. (2007) Clinical development of anti-RANKL therapy. Arthritis Res Ther. 9 Suppl 1: S7.
Leibbrandt A, et al. (2008) RANK/RANKL: regulators of immune responses and bone physiology. Ann N Y Acad Sci. 1143: 123-50.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant mouse TNFSF11/Fc is a disulfide-linked homodimer. The reduced monomer comprises 505 amino acids and has a calculated molecular mass of 56kDa.; The apparent molecular mass of the monomer is approximately 50kDa in SDS-PAGE under reducing c
• Protein Family: Tumor necrosis factor ligand superfamily