REG3D cell lysate

Mouse REG3D HEK293 Overexpression Lysate

Cat. Num. AAA8116681

• Gene Names: Reg3d; INGAP; Ingaprp
• Applications: Western Blot
• Synonyms: REG3D; Mouse REG3D HEK293 Overexpression Lysate; Mouse REG3D HEK293 Cell Lysate (WB positive control); regenerating islet-derived 3 delta; Mouse INGAP Overexpression Lysate; Mouse Ingaprp Overexpression Lysate; Mouse MGC130575 Overexpression Lysate; Mouse Reg3d Overexpression Lysate; REG3D cell lysate

• Host: HEK293 Cells
• Form/Format: 1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).; Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Sequence Positions: 160aa
• Sequence: Met1-Gly175

Applicable Applications for REG3D cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Mouse

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the mouse REG3D isoform 1 (NP_038921.2) extracellular domain (Met 1-Gly 175) was fused with a polyhistidine tag at the C-terminus.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.