CLSTN1 cell lysate
Human CLSTN1 HEK293 Overexpression Lysate
Gene Names
CLSTN1; CSTN1; CDHR12; PIK3CD; FLJ32258; KIAA0911; ALC-ALPHA; XB31alpha; alcalpha1; alcalpha2
Applications
Western Blot
Synonyms
CLSTN1; Human CLSTN1 HEK293 Overexpression Lysate; Human Calsyntenin-1/CLSTN1 (ECD) HEK293 Cell Lysate (WB positive control); calsyntenin 1; Human ALC-ALPHA Overexpression Lysate; Human alcalpha1 Overexpression Lysate; Human alcalpha2 Overexpression Lysate; Human CDHR12 Overexpression Lysate; Human CST-1 Overexpression Lysate; Human CSTN1 Overexpression Lysate; Human PIK3CD Overexpression Lysate; Human XB31alpha Overexpression Lysate; CLSTN1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
842aa
Sequence
Met1-Thr859
Applicable Applications for CLSTN1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human CLSTN1 (NP_001009566.1) (Met1-Thr859) was expressed with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CLSTN1 cell lysate
This Human CLSTN1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CLSTN1 protein from the overexpression lysate was verified.
Product Categories/Family for CLSTN1 cell lysate
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109,793 Da
NCBI Official Full Name
calsyntenin-1 isoform 1
NCBI Official Synonym Full Names
calsyntenin 1
NCBI Official Symbol
CLSTN1
NCBI Official Synonym Symbols
CSTN1; CDHR12; PIK3CD; FLJ32258; KIAA0911; ALC-ALPHA; XB31alpha; alcalpha1; alcalpha2
NCBI Protein Information
calsyntenin-1; alcadein-alpha; alcadein alpha 1; OTTHUMP00000001597; OTTHUMP00000001598; OTTHUMP00000001599; non-classical cadherin XB31alpha; cadherin-related family member 12; non-classical cadherin XB31alpha1; alzheimer-related cadherin-like protein
UniProt Protein Name
Calsyntenin-1
Protein Family
UniProt Gene Name
CLSTN1
UniProt Synonym Gene Names
CS1; KIAA0911
UniProt Entry Name
CSTN1_HUMAN
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Product Notes
The CLSTN1 clstn1 (Catalog #AAA8115614) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 842aa. AAA Biotech's CLSTN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CLSTN1 clstn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Thr85 9. It is sometimes possible for the material contained within the vial of "CLSTN1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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