PRTN3 cell lysate

Human PRTN3 HEK293 Overexpression Lysate

Cat. Num. AAA8115246

• Gene Names: PRTN3; MBN; MBT; NP4; P29; PR3; ACPA; AGP7; NP-4; PR-3; CANCA; C-ANCA
• Applications: Western Blot
• Synonyms: PRTN3; Human PRTN3 HEK293 Overexpression Lysate; Human PRTN3 CHO Cell Lysate (WB positive control); proteinase 3; Human ACPA Overexpression Lysate; Human AGP7 Overexpression Lysate; Human C-ANCA Overexpression Lysate; Human CANCA Overexpression Lysate; Human MBN Overexpression Lysate; Human MBT Overexpression Lysate; Human NP-4 Overexpression Lysate; Human NP4 Overexpression Lysate; Human P29 Overexpression Lysate; Human PR-3 Overexpression Lysate; Human PR3 Overexpression Lysate; PRTN3 cell lysate

• Host: HEK293 Cells
• Form/Format: 1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).; Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Sequence Positions: 240aa
• Sequence: Ile28-Pro256

Applicable Applications for PRTN3 cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.

Sequence Construction

A DNA sequence encoding the human PRTN3 (NP_002768.3, with mutation Ser 203 Ala) (Ile28-Pro256) was expressed with a polyhistide tag at the C-terminus.

Preparation Method

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.