STAT6 cell lysate

Human STAT6 Baculovirus-Insect cells Overexpression Lysate

Gene Names

STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT

Applications

Western Blot

Synonyms

STAT6; Human STAT6 Baculovirus-Insect cells Overexpression Lysate; Human STAT6 Insect Cell Lysate (WB positive control); signal transducer and activator of transcription 6; interleukin-4 induced; Human D12S1644 Overexpression Lysate; Human IL-4-STAT Overexpression Lysate; Human STAT6B Overexpression Lysate; Human STAT6C Overexpression Lysate; STAT6 cell lysate

Ordering
Cat. Num. AAA8115072 For Research Use Only!

Host

Baculovirus-Insect cells

Form/Format

1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Sequence Positions

857aa

Sequence

Met1-Trp847

Applicable Applications for STAT6 cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the human STAT6 isoform 1 (P42226-1) (Met 1-Trp 847) was fused with a polyhistidine tag at the C-terminus.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.

Related Product Information for STAT6 cell lysate

Signal transducer and activator of transcription 6 (STAT6) is a transcription factor that is activated by interleukin-4 (IL-4)-induced tyrosine phosphorylation and mediates most of the IL-4-induced gene expression. STAT6 plays a central role in exerting interleukin-4 (IL-4) mediated biological responses and is found to induce the expression of BCL2L1/BCL-XL, which is responsible for the anti-apoptotic activity of IL4. Transcriptional activation by STAT6 requires the interaction with coactivators like p300 and the CREB-binding protein (CBP). NF-kappaB and tyrosine-phosphorylated Stat6 can directly bind each other in vitro and in vivo, which suggests that the direct interaction between Stat6 and NF-kappaB may provide a basis for synergistic activation of transcription by IL-4 and activators of NF-kappaB.

This Human STAT6 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of STAT6 protein from the overexpression lysate was verified.

Product Categories/Family for STAT6 cell lysate

Apoptosis Transcription Factors and Regulators; Jak-Stat Signaling Pathway

References

Litterst CM, et al. (2001) Transcriptional activation by STAT6 requires the direct interaction with NCoA-1. J Biol Chem. 276 (49): 45713-21.Stutz AM, et al. (1999) Functional synergism of STAT6 with either NF-kappa B or PU.1 to mediate IL-4-induced activation of IgE germline gene transcription. J Immunol. 163 (8): 4383-91.Yang J, et al. (2002) Identification of p100 as a coactivator for STAT6 that bridges STAT6 with RNA polymerase II. EMBO J. 21 (18): 4950-8.

NCBI and Uniprot Product Information

NCBI GI #

296010864

NCBI GeneID

6778

NCBI Accession #

NP_001171549.1

NCBI GenBank Nucleotide #

NM_001178078.1

UniProt Accession #

P42226; Q5FBW5; Q71UP4; A8K316; B7ZA27; F5GXI9

Molecular Weight

94,135 Da

NCBI Official Full Name

signal transducer and activator of transcription 6 isoform 1

NCBI Official Synonym Full Names

signal transducer and activator of transcription 6, interleukin-4 induced

NCBI Official Symbol

STAT6

NCBI Official Synonym Symbols

STAT6B; STAT6C; D12S1644; IL-4-STAT

NCBI Protein Information

signal transducer and activator of transcription 6; STAT, interleukin4-induced; transcription factor IL-4 STAT

UniProt Protein Name

Signal transducer and activator of transcription 6

Protein Family

Signal transducer and transcription activator

UniProt Gene Name

STAT6

UniProt Entry Name

STAT6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

STAT6: transcription factor of the STAT family. Plays a central role in IL4-mediated biological responses. Induces the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. May function in the differentiation of T helper 2 cells and class switch of immunoglobulins. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; cytosol; lipid raft

Molecular Function: identical protein binding; signal transducer activity; protein binding; transcription factor activity; protein phosphatase binding

Biological Process: regulation of transcription from RNA polymerase II promoter; T-helper 1 cell lineage commitment; negative regulation of T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interferon type I production; innate immune response; mammary gland epithelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of isotype switching to IgE isotypes; regulation of cell proliferation

Research Articles on STAT6

1. PPIs, in concentrations achieved in blood with conventional dosing, significantly inhibit IL-4-stimulated eotaxin-3 expression in EoE esophageal cells and block STAT6 binding to the promoter.

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Product Notes
The STAT6 stat6 (Catalog #AAA8115072) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 857aa. AAA Biotech's STAT6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the STAT6 stat6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Trp84 7. It is sometimes possible for the material contained within the vial of "STAT6, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.