SRPK3 cell lysate
Human SRPK3 Baculovirus-Insect cells Overexpression Lysate
Gene Names
SRPK3; MSSK1; STK23; MSSK-1
Applications
Western Blot
Synonyms
SRPK3; Human SRPK3 Baculovirus-Insect cells Overexpression Lysate; Human STK23/MSSK1/SRPK3 Insect Cell Lysate (WB positive control); SRSF protein kinase 3; Human MGC102944 Overexpression Lysate; Human MSSK-1 Overexpression Lysate; Human MSSK1 Overexpression Lysate; Human STK23 Overexpression Lysate; SRPK3 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
803aa
Sequence
Met1-Pro566
Applicable Applications for SRPK3 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding full length of human SRPK3 isoform 2 (NP_001164231.1) (Met 1-Pro 566) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SRPK3 cell lysate
Serine/threonine-protein kinase SRPK3, also known as Muscle-specific serine kinase 1, Serine/arginine-rich protein-specific kinase 3, SR-protein-specific kinase 3, Serine/threonine-protein kinase 23, MSSK-1, SRPK3 and MSSK1, is a member of the protein kinase superfamily and CMGC Ser/Thr protein kinase family. SRPK3 is a protein kinase belonging to serine/arginine protein kinases (SRPK) family, which phosphorylates serine/arginine repeat-containing proteins, and is controlled by a muscle-specific enhancer directly regulated by MEF2. SRPK3/MSSK1 contains one protein kinase domain. SRPK3/MSSK1 is exclusively expressed in skeletal and heart muscle. It is required for normal muscle development. Myocyte enhancer factor 2 (MEF2) plays essential roles in transcriptional control of muscle development. Normal muscle growth and homeostasis require MEF2-dependent signaling by SRPK3.
This Human SRPK3 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of SRPK3 protein from the overexpression lysate was verified.
This Human SRPK3 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of SRPK3 protein from the overexpression lysate was verified.
References
Greenman C., et al., 2007, Nature 446:153-8. Xu, Y. et al., 2011, Mol Biol Rep. 38 (5):2903-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,014 Da
NCBI Official Full Name
SRSF protein kinase 3 isoform 2
NCBI Official Synonym Full Names
SRSF protein kinase 3
NCBI Official Symbol
SRPK3
NCBI Official Synonym Symbols
MSSK1; STK23; MSSK-1
NCBI Protein Information
SRSF protein kinase 3; SFRS protein kinase 3; serine/threonine kinase 23; SR-protein-specific kinase 3; muscle-specific serine kinase 1; serine/threonine-protein kinase 23; serine/threonine-protein kinase SRPK3; serine arginine rich protein-specific kinas
UniProt Protein Name
SRSF protein kinase 3
Protein Family
UniProt Gene Name
SRPK3
UniProt Synonym Gene Names
MSSK1; STK23; MSSK-1; SR-protein-specific kinase 3
UniProt Entry Name
SRPK3_HUMAN
Similar Products
Product Notes
The SRPK3 srpk3 (Catalog #AAA8114897) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 803aa. AAA Biotech's SRPK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SRPK3 srpk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro56 6. It is sometimes possible for the material contained within the vial of "SRPK3, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
