Factor IX Cell Lysate | F9 cell lysate
Human Factor IX HEK293 Overexpression Lysate
Gene Names
F9; FIX; P19; PTC; HEMB; THPH8; F9 p22
Applications
Western Blot
Synonyms
Factor IX; Human Factor IX HEK293 Overexpression Lysate; Human Coagulation Factor IX/FIX/F9 HEK293 Cell Lysate (WB positive control); coagulation factor IX; Human Coagulation factor 9 Overexpression Lysate; Human Coagulation factor IX Overexpression Lysate; Human FIX Overexpression Lysate; Human HEMB Overexpression Lysate; Human P19 Overexpression Lysate; Human PTC Overexpression Lysate; Human THPH8 Overexpression Lysate; F9 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
444aa
Sequence
Met1-Thr461
Applicable Applications for F9 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human F9 (AAB59620.1) (Met 1-Thr 461) was expressed, fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for F9 cell lysate
Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX/F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one peptidase S1 domain. Coagulation factor IX/F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX/F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX/F9 are also the cause of recessive X-linked hemophilia B (HEMB) which also known as Christmas disease.
This Human Factor IX overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Factor IX protein from the overexpression lysate was verified.
This Human Factor IX overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Factor IX protein from the overexpression lysate was verified.
References
Onay U.V., et al., 2003, Br. J. Haematol. 120:656-659. Vidal F., et al., 2000, Br. J. Haematol. 111:549-551. Simioni P., et al., 2009, N. Engl. J. Med. 361:1671-1675. Espinos C., et al., 2009, Haematologica 88:235-236.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
47,618 Da
NCBI Official Full Name
factor IX
NCBI Official Synonym Full Names
coagulation factor IX
NCBI Official Symbol
F9
NCBI Official Synonym Symbols
FIX; P19; PTC; HEMB; THPH8; F9 p22
NCBI Protein Information
coagulation factor IX
Protein Family
NCBI Description
This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Sep 2015]
Research Articles on F9
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Product Notes
The F9 (Catalog #AAA8114553) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 444aa. AAA Biotech's Factor IX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the F9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Thr46 1. It is sometimes possible for the material contained within the vial of "Factor IX, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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