respiratory syncytial virus (RSV) (A, rsb1734) glycoprotein G/RSV-G Cell Lysate | RSV-G cell lysate
Human respiratory syncytial virus (RSV) (A, rsb1734) glycoprotein G/RSV-G Insect Cell Lysate (WB positive control)
Applications
Western Blot
Synonyms
respiratory syncytial virus (RSV) (A; rsb1734) glycoprotein G/RSV-G; Human respiratory syncytial virus (RSV) (A; rsb1734) glycoprotein G/RSV-G Insect Cell Lysate (WB positive control); RSV G Overexpression Lysate; RSV-G cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
242aa
Sequence
Asn66-Arg297
Applicable Applications for RSV-G cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human respiratory syncytial viru
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the glycoprotein G extracellular domain (Asn 66-Arg297) of human respiratory syncytial virus A (93% homologous with strain rsb1734) (P27022-1) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for RSV-G cell lysate
Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. HRSV G protein is a type II glycoprotein of 289-299 amino acids (depending on the virus strain) with a signal/anchor hydrophobic domain and is extensively modified by the addition of both N-and O-linked oligosaccharides to achieve the mature form of 8-9 kDa. The C-terminal ectodomain of the G protein has a central region and four cysteines which are conserved in all HRSV isolates and have been proposed as the putative receptor binding site. The G protein mediates attachment of the virus to the host cell membrane by interacting with heparan sulfate, initiating the infection. As similar to mucins in amino acid compositions, the RSV G protein can interact with host CX3CR1, the receptor for the CX3C chemokine fractalkine, and thus modulates the immune response and facilitate infection. Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors. Unlike the other paramyxovirus attachment proteins, HRSV-G lacks both neuraminidase and hemagglutinating activities.
This RSV RSV Glycoprotein G overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of RSV Glycoprotein G protein from the overexpression lysate was verified.
This RSV RSV Glycoprotein G overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of RSV Glycoprotein G protein from the overexpression lysate was verified.
References
Martin-Gallardo A. et al., 1993, J Gen Virol. 74 : 453-8. Jose AM. et al., 1997, J Gen Virol. 78: 2411-8. Feldman SA. et al., 1999, J Virol. 73: 6610-7. Garcia-Beato R. et al., 2000, J Gen Virol. 81: 919-27. Zlateva KT. et al., 2004, J Virol. 78: 4675-83. Trento A. et al., 2006, J Virol. 80: 975-84.
NCBI and Uniprot Product Information
Uniprot Description
Function: Attaches the virion to the host cell membrane by interacting with heparan sulfate, initiating the infection. Interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, to modulate the immune response and facilitate infection. Unlike the other paramyxovirus attachment proteins, lacks both neuraminidase and hemagglutinating activities
By similarity.Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors
By similarity.
Subunit structure: Homooligomer. Interacts (via N-terminus) with protein M. Interacts with protein F; this interaction occurs on the surface of infected cells. Interacts with protein SH. Interacts with host CX3CR1; this interaction modulates host immune response
By similarity.
Subcellular location: Virion membrane. Host cell surface
By similarity. Isoform Secreted glycoprotein G: Secreted.
Domain: Contains a linear heparin binding domain essential for virus attachment to the host
By similarity.
Post-translational modification: May carry a lot of separate O-linked carbohydrate chains distributed among serine and threonine residues
By similarity.Palmitoylated
By similarity.
Sequence similarities: Belongs to the pneumoviruses glycoprotein G family.
Similar Products
Product Notes
The RSV-G g (Catalog #AAA8114387) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 242aa. AAA Biotech's respiratory syncytial virus (RSV) (A, rsb1734) glycoprotein G/RSV-G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the RSV-G g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Asn66-Arg2 97. It is sometimes possible for the material contained within the vial of "respiratory syncytial virus (RSV) (A, rsb1734) glycoprotein G/RSV-G, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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