Midkine Cell Lysate | MDK cell lysate

Human Midkine Baculovirus-Insect cells Overexpression Lysate

Cat. Num. AAA8113648

• Gene Names: MDK; MK; ARAP; NEGF2
• Applications: Western Blot
• Synonyms: Midkine; Human Midkine Baculovirus-Insect cells Overexpression Lysate; Human Midkine/MDK Insect Cell Lysate (WB positive control); midkine (neurite growth-promoting factor 2); Human ARAP Overexpression Lysate; Human MK Overexpression Lysate; Human NEGF2 Overexpression Lysate; MDK cell lysate

• Host: Baculovirus-Insect cells
• Form/Format: 1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).; Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Sequence Positions: 123aa
• Sequence: Met1-Asp143

Applicable Applications for MDK cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the human MDK (P21741) (Met 1-Asp 143) was expressed and purified.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.