Rabbit anti-Rat IL4R Monoclonal Antibody | anti-IL4R antibody

Recombinant Anti-IL4R Antibody (APC), Rabbit Monoclonal

Cat. Num. AAA8112810

• Gene Names: Il4r; Il4ra
• Reactivity: Rat
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: IL4R; Monoclonal Antibody; Recombinant Anti-IL4R Antibody (APC); Rabbit Monoclonal; IL4R Antibody (APC); Rabbit MAb; interleukin 4 receptor; Anti-IL4R Antibody; Anti-Il4ra Antibody; anti-IL4R antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 002
• Specificity: Rat IL4R
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.03%ProClin300
• Concentration: 5ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-IL4R antibody: Flow Cytometry (FC/FACS/FCM)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Rat IL4R Protein
• Conjugation: APC
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Rat IL4R (rR IL4R) and conjugated with APC under optimum conditions, the unreacted APC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze!

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of rat IL4R expression on SD rat splenocytes. Cells were stained with APC-conjugated anti-rat IL4R. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-IL4R antibody

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alters the behavior of the cell. Some CD proteins do not take part in the cell signal process but have other functions such as cell adhesion. CD124, also known as the interleukin 4 receptor (IL4R), is a typeI  transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to a soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.

Product Categories/Family for anti-IL4R antibody

AKT Signaling Pathway

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.

NCBI and Uniprot Product Information

• NCBI GI #: 158534071
• NCBI GeneID: 25084
• NCBI Accession #: NP_596871.2
• NCBI GenBank Nucleotide #: NM_133380.2
• UniProt Accession #: Q63257; Q9R1W8; Q9WTM8
• Molecular Weight: 26,001 Da
• NCBI Official Full Name: interleukin-4 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 4 receptor
• NCBI Official Symbol: Il4r
• NCBI Official Synonym Symbols: Il4ra
• NCBI Protein Information: interleukin-4 receptor subunit alpha
• UniProt Protein Name: Interleukin-4 receptor subunit alpha
• Protein Family: Interleukin-4 receptor
• UniProt Gene Name: Il4r
• UniProt Synonym Gene Names: Il4ra; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA

NCBI Description

a receptor for the cytokine IL-4 [RGD, Feb 2006]

Uniprot Description

IL4R: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Belongs to the type I cytokine receptor family. Type 4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1q36

Cellular Component: extracellular space; integral component of membrane; plasma membrane; receptor complex

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein heterodimerization activity

Biological Process: cytokine-mediated signaling pathway; defense response to protozoan; immune response; immunoglobulin mediated immune response; inflammatory response; negative regulation of T-helper 1 cell differentiation; ovulation; positive regulation of immunoglobulin production; positive regulation of macrophage activation; positive regulation of mast cell degranulation; positive regulation of T-helper 2 cell differentiation; production of molecular mediator of acute inflammatory response; regulation of cell proliferation; response to estrogen