Rabbit anti-Mouse IGFBP2 Polyclonal Antibody | anti-IGFBP2 antibody

Anti-IGFBP2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8112342

• Gene Names: Igfbp2; IBP-2; Igfbp-2; AI255832; mIGFBP-2
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: IGFBP2; Polyclonal Antibody; Anti-IGFBP2 Antibody; Rabbit Polyclonal; IGFBP-2/IGFBP2 Antibody; Rabbit PAb; Antigen Affinity Purified; insulin-like growth factor binding protein 2; 36kDa; Anti-AI255832 Antibody; Anti-IBP-2 Antibody; Anti-Igfbp-2 Antibody; Anti-mIGFBP-2 Antibody; anti-IGFBP2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse IGFBP-2/IGFBP2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IGFBP2 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:500-1:2000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Mouse IGFBP-2/IGFBP2 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse IGFBP-2/IGFBP2 (rM IGFBP-2/IGFBP2). IGFBP-2/IGFBP2 specific IgG was purified by Mouse IGFBP-2/IGFBP2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse IGFBP2 in mouse kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(Mouse IGFBP2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-Mouse IGFBP2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-Mouse IGFBP2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 37 kDa)

Western Blot (WB)

(Anti-IGFBP2- rabbit polyclonal antibody at 1:500 dilutionLane A: SH-SY5Y Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:36 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-IGFBP2 antibody

IGFBP-2, also known as IGFBP2, is an insulin-like growth factor-binding protein (IGFBP). IGFBPs prolong the half-life of the IGFs, control bioavailability, activity, and distribution of insulin-like growth factor (IGF) through high-affinity IGFBP/IGF complexes. Six high-affinity IGF-binding proteins (IGFBP-1 to -6) have been identified. The six IGFBPs are structurally related but encoded by distinct genes. IGFBPs have a high affinity for IGFs. Some members of the IGFBP family have been consistently shown to inhibit IGF actions by preventing them from gaining access to the IGF receptors, while others potentiate IGF actions by facilitating the ligand-receptor interaction. IGFBP-2 is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to the potential involvement of IGFBP-2 in tumorigenesis. It contains 1 IGFBP N-terminal domain and 1 thyroglobulin type-1 domain. It inhibits IGF-mediated growth and developmental rates.

References

Han VK, et al. (1996) The expression of insulin-like growth factor (IGF) and IGF-binding protein (IGFBP) genes in the human placenta and membranes: evidence for IGF-IGFBP interactions at the feto-maternal interface. J Clin Endocrinol Metab. 81(7): 2680-93.Binkert C, et al. (1989) Cloning, sequence analysis and expression of a cDNA encoding a novel insulin-like growth factor binding protein (IGFBP-2). EMBO J. 8(9):2497-502.Wolf E, et al. (2000) Effects of IGFBP-2 overexpression in vitro and in vivo. Pediatr Nephrol. 14 (7):572-8.

NCBI and Uniprot Product Information

• NCBI GI #: 31982260
• NCBI GeneID: 16008
• NCBI Accession #: NP_032368.2
• NCBI GenBank Nucleotide #: NM_008342.3
• UniProt Accession #: P47877; Q61722; Q91VK7
• Molecular Weight: 32,847 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 2 isoform 1
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 2
• NCBI Official Symbol: Igfbp2
• NCBI Official Synonym Symbols: IBP-2; Igfbp-2; AI255832; mIGFBP-2
• NCBI Protein Information: insulin-like growth factor-binding protein 2
• UniProt Protein Name: Insulin-like growth factor-binding protein 2
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: Igfbp2
• UniProt Synonym Gene Names: Igfbp-2; IBP-2; IGF-binding protein 2; IGFBP-2; mIGFBP-2
• UniProt Entry Name: IBP2_MOUSE

NCBI Description

The protein encoded by this gene is one of several similar proteins that bind insulin-like growth factors I and II (Igf-I and Igf-II). The encoded protein can be secreted into the bloodstream, where it binds Igf-I and Igf-II with high affinity, or it can remain intracellular, interacting with many different ligands. Two transcript variants, one encoding a secreted isoform and the other encoding a nonsecreted isoform, have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

IGFBP2: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Binds IGF2 more than IGF1.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; apical plasma membrane; extracellular region; cytoplasmic vesicle

Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; growth factor binding; insulin-like growth factor II binding

Biological Process: regulation of insulin-like growth factor receptor signaling pathway; regulation of growth; regulation of cell growth; positive regulation of activated T cell proliferation