Rabbit anti-Mouse Cathepsin A Polyclonal Antibody | anti-CTSA antibody

Anti-Cathepsin A Antibody, Rabbit Polyclonal

Cat. Num. AAA8111422

• Gene Names: Ctsa; PPCA; Ppgb; AU019505
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Cathepsin A; Polyclonal Antibody; Anti-Cathepsin A Antibody; Rabbit Polyclonal; Cathepsin A/CTSA Antibody; Rabbit PAb; Antigen Affinity Purified; cathepsin A; Anti-AU019505 Antibody; Anti-PPCA Antibody; Anti-Ppgb Antibody; anti-CTSA antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Cathepsin A/CTSA
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CTSA antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 4-6uL/mg of lysate
• Immunogen: Recombinant Mouse Cathepsin A/CTSA protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse Cathepsin A/CTSA. Cathepsin A/CTSA specific IgG was purified by Mouse Cathepsin A/CTSA affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(Mouse CTSA was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate2 uL anti-Mouse CTSA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CTSA rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 30 kDa)

Western Blot (WB)

(Anti-CTSA rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:28 kDa)

Related Product Information for anti-CTSA antibody

Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with beta-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation. Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. It was recently identified in human platelets as deamidase. In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions. It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases. Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase. Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation.

Product Categories/Family for anti-CTSA antibody

Serine Proteases and Regulators

References

Hiraiwa M. (1999) Cathepsin A/protective protein: an unusual lysosomal multifunctional protein. Cell Mol Life Sci. 56(11-12): 894-907.Yoshida T, et al. (2006) Comparative analysis of binding energy of chymostatin with human cathepsin A and its homologous proteins by molecular orbital calculation. J Chem Inf Model. 46(5): 2093-103.Seyrantepe V, et al. (2008) Enzymatic activity of lysosomal carboxypeptidase (cathepsin) A is required for proper elastic fiber formation and inactivation of endothelin-1. Circulation. 117(15): 1973-81.

NCBI and Uniprot Product Information

• NCBI GI #: 84042523
• NCBI GeneID: 19025
• NCBI Accession #: NP_001033581.1
• NCBI GenBank Nucleotide #: NM_001038492.2
• UniProt Accession #: P16675; Q8VEF6
• Molecular Weight: 53,844 Da
• NCBI Official Full Name: lysosomal protective protein isoform b preproprotein
• NCBI Official Synonym Full Names: cathepsin A
• NCBI Official Symbol: Ctsa
• NCBI Official Synonym Symbols: PPCA; Ppgb; AU019505
• NCBI Protein Information: lysosomal protective protein
• UniProt Protein Name: Lysosomal protective protein
• Protein Family: Lysosomal protective protein
• UniProt Gene Name: Ctsa
• UniProt Synonym Gene Names: Ppgb; PPCA

NCBI Description

This gene encodes a glycoprotein with deamidase, esterase and carboxypeptidase activities. The encoded protein associates with and provides a protective function to the lysosomal enzymes beta-galactosidase and neuraminidase. Deficiency of the related gene in humans results in galactosialidosis. The proprotein is processed into two shorter chains. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

CTSA: Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins. Defects in CTSA are the cause of galactosialidosis (GSL). A lysosomal storage disease associated with a combined deficiency of beta-galactosidase and neuraminidase, secondary to a defect in cathepsin A. All patients have clinical manifestations typical of a lysosomal disorder, such as coarse facies, cherry red spots, vertebral changes, foam cells in the bone marrow, and vacuolated lymphocytes. Three phenotypic subtypes are recognized. The early infantile form is associated with fetal hydrops, edema, ascites, visceromegaly, skeletal dysplasia, and early death. The late infantile type is characterized by hepatosplenomegaly, growth retardation, cardiac involvement, and a normal or mildly affected mental state. The juvenile/adult form is characterized by myoclonus, ataxia, angiokeratoma, mental retardation, neurologic deterioration, absence of visceromegaly, and long survival. Belongs to the peptidase S10 family.

Protein type: EC 3.4.16.5; Endoplasmic reticulum; Mitochondrial; Protease

Chromosomal Location of Human Ortholog: 2 H3|2 85.27 cM

Cellular Component: intracellular membrane-bound organelle; lysosomal membrane; membrane; mitochondrion; nucleoplasm

Molecular Function: glycoprotein binding; protein binding; serine carboxypeptidase activity

Biological Process: proteolysis; proteolysis involved in cellular protein catabolic process; regulation of protein stability