Rabbit anti-Human POLR2A Polyclonal Antibody | anti-POLR2A antibody

Anti-POLR2A Antibody, Rabbit Polyclonal

Cat. Num. AAA8108782

• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: POLR2A; Polyclonal Antibody; Anti-POLR2A Antibody; Rabbit Polyclonal; POLR2A Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-hRPB220 Antibody; Anti-hsRPB1 Antibody; Anti-POLR2 Antibody; Anti-POLRA Antibody; Anti-RPB1 Antibody; Anti-RPBh1 Antibody; Anti-RpIILS Antibody; Anti-RPO2 Antibody; Anti-RPOL2 Antibody; anti-POLR2A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human POLR2A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-POLR2A antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:100-1:500
• Immunogen: E Coli-derived Human POLR2A fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human POLR2A fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-POLR2A rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:192 kDa Observed band size:250 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of POLR2A in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human POLR2A polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

Related Product Information for anti-POLR2A antibody

POLR2A is identified as such a gene that is almost always co-deleted with TP53 in human cancers. It encodes the largest and catalytic subunit of the RNA polymerase II complex, which is specifically inhibited by alpha-amanitin. The carboxy-terminal domain (CTD) of the RNA polymerase II (RNAP II) subunit POLR2A is a platform for modifications specifying the recruitment of factors that regulate transcription, mRNA processing, and chromatin remodeling. Scientists identified POLR2A in the TP53-neighbouring region as a collateral vulnerability target in TNBC tumors, suggesting that its inhibition via small interfering RNA (siRNA) may be an amenable approach for TNBC targeted treatment. Transcription start sites (TSSs) with a motif-linked occupancy of H2AFZ, a component of positioned nucleosomes, showed a distinct pattern of RNA Polymerase II (POLR2A) occupancy and TSS flank transcription in primary and transformed cells.

References

Zhao DY, et al. (2016) Smn and symmetric arginine dimethylation of rna polymerase ii c-terminal domain control termination. Nature 529 (7584): 48-53.Xu J, et al. (2020) Author correction: Precise targeting of polr2a as a therapeutic strategy for human triple negative breast cancer. Nat Nanotechnol 15 (4): 342.Liu Y, et al. (2015) Tp53 loss creates therapeutic vulnerability in colorectal cancer. Nature 520 (7549): 697-701.Datta S, et al. (2019) Distinct DNA sequence preference for histone occupancy in primary and transformed cells. Cancer Inform 18 1176935119843835.