Immunofluorescence (IF)
(Immunofluorescence staining of CLIP4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CLIP4 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Vesicles.)
Rabbit anti-Human CLIP4 Polyclonal Antibody | anti-CLIP4 antibody
Anti-CLIP4 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
CLIP4; Polyclonal Antibody; Anti-CLIP4 Antibody; Rabbit Polyclonal; CLIP4 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-RSNL2 Antibody; anti-CLIP4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CLIP4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 100mM Tris, 100mM Glycine, 10mM Nacl, 0.03% Proclin300, pH8.0
Applicable Applications for anti-CLIP4 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human CLIP4 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human CLIP4 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of CLIP4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CLIP4 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Vesicles.)
Immunofluorescence (IF)
(Immunofluorescence staining of CLIP4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CLIP4 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Vesicles.)
Related Product Information for anti-CLIP4 antibody
CLIP4, also known as UBASH3A or TULA, is a member of the T-cell ubiquitin ligand family and suppresses T-cell signaling. CLIP4 can facilitate growth factor withdrawal-induced apoptosis in T-cells and promote the accumulation of activated various target receptors, such as T-cell receptors, epidermal growth factor receptor, and platelet-derived growth factor beta-receptor, which can induce cell invasiveness and metastasis. CLIP4 were found to activate Syk, one of the protein tyrosine kinase, and Syk expression were known to be linked with cell motility and increased cell migration. Also CLIP4 found to be capable of inhibiting c-Cbl which downregulates EGF receptor and activates EGFR signaling pathway which is known to be associated with EMT pathway. Close family members of CLIP4/UBASH3B were also found to be associated with metastasis in breast cancer, and CLIP4 expression could stimulate tumor metastasis in some tumor types.
References
Ahn J, et al. (2016) Foxc2 and clip4 : A potential biomarker for synchronous metastasis of <=7-cm clear cell renal cell carcinomas.
Oncotarget 7 (32): 51423-51434.
Oncotarget 7 (32): 51423-51434.
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Product Notes
The CLIP4 (Catalog #AAA8108341) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CLIP4 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLIP4 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the CLIP4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLIP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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