Mouse anti-Human LAMP2 Monoclonal Antibody | anti-LAMP2 antibody

Anti-LAMP2 Antibody, Mouse Monoclonal

Cat. Num. AAA8107001

• Gene Names: LAMP2; LAMPB; CD107b; LAMP-2; LGP110
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: LAMP2; Monoclonal Antibody; Anti-LAMP2 Antibody; Mouse Monoclonal; LAMP2/CD107b Antibody; Mouse MAb; lysosomal-associated membrane protein 2; Anti-CD107b Antibody; Anti-LAMP-2 Antibody; Anti-LAMPB Antibody; Anti-LGP110 Antibody; anti-LAMP2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 05
• Specificity: Human LAMP2/CD107b
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LAMP2 antibody: Flow Cytometry (FC/FACS/FCM)
• Application Notes: FC/FACS/FCM: 1:25-1:100
• Immunogen: Recombinant Human LAMP2/CD107b Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LAMP2/CD107b (rh LAMP2/CD107b). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human LAMP2(CD107b) expression on Jurkat cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human LAMP2(CD107b), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-LAMP2 antibody

LAMP2 (Lysosomal Associated Membrane Protein 2) is a Protein Coding gene. LAMP2, also known as CD107b (Cluster of Differentiation 107b), is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. In humans, LAMP2, the causative gene of Danon disease, located on chromosome Xq24, encodes the lysosome-associated membrane protein-2 (LAMP-2). LAMP-2 deficiency, or Danon disease, is a rare X-linked lysosomal disease characterized by cardiomyopathy, vacuolar myopathy, and mental retardation. LAMP2 cardiomyopathy is an X-linked and highly progressive myocardial storage disorder associated with diminished survival, which clinically resembles sarcomeric hypertrophic cardiomyopathy.

References

Maron BJ, et al. (2010) Profound left ventricular remodeling associated with LAMP2 cardiomyopathy. Am J Cardiol. 106(8): 1194-6.Di Blasi C, et al. (2008) Danon disease: a novel LAMP2 mutation affecting the pre-mRNA splicing and causing aberrant transcripts and partial protein expression. Neuromuscul Disord. 18(12): 962-6.Echaniz-Laguna A, et al. (2006) Novel Lamp-2 gene mutation and successful treatment with heart transplantation in a large family with Danon disease. Muscle Nerve. 33(3): 393-7.

NCBI and Uniprot Product Information

• NCBI GI #: 7669503
• NCBI GeneID: 3920
• NCBI Accession #: NP_054701.1
• NCBI GenBank Nucleotide #: NM_013995.2
• UniProt Accession #: P13473; Q16641; Q6Q3G8; Q96J30; Q99534; Q9UD93; A8K4X5; D3DTF0
• Molecular Weight: 45,170 Da
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 2 isoform B
• NCBI Official Synonym Full Names: lysosomal-associated membrane protein 2
• NCBI Official Symbol: LAMP2
• NCBI Official Synonym Symbols: LAMPB; CD107b; LAMP-2; LGP110
• NCBI Protein Information: lysosome-associated membrane glycoprotein 2; CD107 antigen-like family member B
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 2
• Protein Family: Lysosome-associated membrane glycoprotein
• UniProt Gene Name: LAMP2
• UniProt Synonym Gene Names: LAMP-2; Lysosome-associated membrane protein 2
• UniProt Entry Name: LAMP2_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

LAMP2: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. Defects in LAMP2 are the cause of Danon disease (DAND); also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. Belongs to the LAMP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: phagocytic vesicle membrane; membrane; lysosome; lysosomal membrane; late endosome membrane; late endosome; plasma membrane; integral to membrane; platelet dense granule membrane

Molecular Function: protein domain specific binding; enzyme binding

Biological Process: platelet activation; platelet degranulation; protein stabilization; blood coagulation

Disease: Danon Disease