Western Blot (WB)
(Anti-ERN1 rabbit polyclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:110 kDaObserved band size:110 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human IRE1 Polyclonal Antibody | anti-ERN1 antibody
Anti-IRE1 Antibody, Rabbit Polyclonal
Gene Names
ERN1; IRE1; IRE1P; IRE1a; hIRE1p
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
IRE1; Polyclonal Antibody; Anti-IRE1 Antibody; Rabbit Polyclonal; ERN1/IRE1 Antibody; Rabbit PAb; Antigen Affinity Purified; endoplasmic reticulum to nucleus signaling 1; Anti-hIRE1p Antibody; Anti-IRE1a Antibody; Anti-IRE1P Antibody; anti-ERN1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ERN1/IRE1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ERN1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
ELISA: 1:25,000-1:50,000
Immunogen
Recombinant Human ERN1/IRE1 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human ERN1/IRE1 (rh ERN1/IRE1). ERN1/IRE1 specific IgG was purified by Human ERN1/IRE1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-ERN1 rabbit polyclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:110 kDaObserved band size:110 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-ERN1 rabbit polyclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:110 kDaObserved band size:110 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-ERN1 antibody
Endoplasmic reticulum stress and hypoxia are necessary components of malignant tumors growth and suppression of ERN1 (from endoplasmic reticulum to nuclei-1) signalling pathway, which is linked to the apoptosis and cell death processes, significantly decreases proliferative processes. An enhanced expression of TP53 gene in ERN1 knockdown glioma cells correlates with the decreased level of ubiquitin ligase MDM2 and increased expression level of USP7 which deubiquitinates TP53 and MDM2 and induces TP53-dependent cell growth repression and apoptosis. Thus, the expression of genes encoding TP53 and related to TP53 factors depends upon the endoplasmic reticulum stress signaling as well as on hypoxia, and correlates with suppression of glioma growth under ERN1 knockdown. The dependence of insulin-like growth binding proteins as well as IGF2BP3 and HTRA1 gene expressions in U87 glioma cells on ERN1 signaling enzyme function and hypoxia, indicating its participation in the regulation of metabolic and proliferative processes via IGF/INS receptors, because endoplasmic reticulum stress is an important component of tumor growth and metabolic diseases.
References
Trentmann, S.M. et al., 2000, Plant Mol Biol. 44 (1):11-25. Iwawaki T., et al., 2001, Nat. Cell Biol. 3:158-64. Liu C.Y., et al., 2003, J. Biol. Chem. 278:17680-7. Oppermann F.S., et al., 2009, Mol. Cell. Proteomics 8:1751-64.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
977
NCBI Official Full Name
Serine/threonine-protein kinase/endoribonuclease IRE1
NCBI Official Synonym Full Names
endoplasmic reticulum to nucleus signaling 1
NCBI Official Symbol
ERN1
NCBI Official Synonym Symbols
IRE1; IRE1P; IRE1a; hIRE1p
NCBI Protein Information
serine/threonine-protein kinase/endoribonuclease IRE1; ERN1; ire1-alpha; inositol-requiring 1; ER to nucleus signalling 1; inositol-requiring enzyme 1; inositol-requiring protein 1; endoplasmic reticulum-to-nucleus signaling 1
UniProt Protein Name
Serine/threonine-protein kinase/endoribonuclease IRE1
UniProt Gene Name
ERN1
UniProt Synonym Gene Names
hIRE1p; IRE1a
UniProt Entry Name
ERN1_HUMAN
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Product Notes
The ERN1 ern1 (Catalog #AAA8105971) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IRE1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IRE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000. Researchers should empirically determine the suitability of the ERN1 ern1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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