Mouse anti-Human RON/CD136 Monoclonal Antibody | anti-MST1R antibody

Anti-RON/CD136 Antibody (PE), Mouse Monoclonal

Cat. Num. AAA8105483

• Gene Names: MST1R; RON; PTK8; CD136; CDw136
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: RON/CD136; Monoclonal Antibody; Anti-RON/CD136 Antibody (PE); Mouse Monoclonal; CD136/MST1R Antibody (PE); Mouse MAb; macrophage stimulating 1 receptor; Anti-CD136 Antibody; Anti-CDw136 Antibody; Anti-PTK8 Antibody; Anti-RON Antibody; anti-MST1R antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 03
• Specificity: Human CD136/MST1R
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 10ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-MST1R antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human CD136/MST1R Protein
• Conjugation: PE
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD136/MST1R (rh CD136/MST1R) and conjugated with PE under optimum conditions, the unreacted PE was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human CD136 expression on SW480 cells. Cells were stained with PE-conjugated anti-Human CD136. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-MST1R antibody

The tyrosine kinase receptor, macrophage-stimulating 1 receptor (MST1R), a c-met-related tyrosine kinase, also known as the Ron receptor or CD136, controls cell survival and motility programs related to invasive growth. As the tyrosine kinase receptor is comprised of an extracellular domain, MST1R protein contains the ligand-binding pocket and an intracellular region where the kinase domain is located. MST1R signaling may be involved in the regulation of macrophage and T-lymphocyte activation in vivo during injury. This assessment of gene expression indicates the importance of genetic factors in contributing to lung injury and points to strategies for intervention in the progression of inflammatory diseases. It had been shown that MST1R/CD136 plays a critical role in Ni-induced lung injury in mice. The overexpression of MSP, MT-SP1, and MST1R was a strong independent indicator of both metastasis and death in human breast cancer patients and significantly increased the accuracy of an existing gene expression signature for poor prognosis. Stimulation of MST1R leads to its transphosphorylation and the ultimate activation of numerous intracellular signaling pathways, such as the classical mitogen-activated protein kinase pathway, the phosphatidylinositol (PI)3-kinase pathway, and the JNK pathway.

Product Categories/Family for anti-MST1R antibody

Receptor Tyrosine Kinases (RTKs)

References

Ronsin C, et al. (1993) A novel putative receptor protein tyrosine kinase of the met family. Oncogene. 8: 1195-1202.McDowell SA, et al. (2002) The role of the receptor tyrosine kinase Ron in nickel-induced acute lung injury. Am J Respir Cell Mol Biol. 26(1): 99-104.Angeloni D, et al. (2003) C to A single nucleotide polymorphism in intron 18 of the human MST1R (RON) gene that maps at 3p21.3. Mol Cell Probes. 17(2-3): 55-7.Mallakin A, et al. (2006) Gene expression profiles of Mst1r-deficient mice during nickel-induced acute lung injury. Am J Respir Cell Mol Biol. 34(1): 15-27.Welm AL, et al. (2007) The macrophage-stimulating protein pathway promotes metastasis in a mouse model for breast cancer and predicts poor prognosis in humans. Proc Natl Acad Sci U S A. 104(18): 7570-5.

NCBI and Uniprot Product Information

• NCBI GI #: 349732251
• NCBI GeneID: 4486
• NCBI Accession #: NP_001231866.1
• NCBI GenBank Nucleotide #: NM_001244937.1
• UniProt Accession #: Q04912; B5A944; B5A945; B5A946; B5A947
• Molecular Weight: 152,271 Da
• NCBI Official Full Name: macrophage-stimulating protein receptor isoform 2
• NCBI Official Synonym Full Names: macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
• NCBI Official Symbol: MST1R
• NCBI Official Synonym Symbols: RON; PTK8; CD136; CDw136
• NCBI Protein Information: macrophage-stimulating protein receptor; p185-Ron; MSP receptor; RON variant 21; RON variant E2E3; MST1R variant RON30; MST1R variant RON62; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4; c-met-related tyrosine
• UniProt Protein Name: Macrophage-stimulating protein receptor
• Protein Family: Macrophage-stimulating protein receptor
• UniProt Gene Name: MST1R
• UniProt Synonym Gene Names: PTK8; RON; MSP receptor
• UniProt Entry Name: RON_HUMAN

NCBI Description

This gene encodes a cell surface receptor for macrophage-stimulating protein (MSP) with tyrosine kinase activity. The mature form of this protein is a heterodimer of disulfide-linked alpha and beta subunits, generated by proteolytic cleavage of a single-chain precursor. The beta subunit undergoes tyrosine phosphorylation upon stimulation by MSP. This protein is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung, and together with MSP, thought to be involved in host defense. Alternatively spliced transcript variants encoding different isoforms with different structural and biochemical properties have been described (PMID:8816464). [provided by RefSeq, Oct 2011]

Uniprot Description

Ron: a receptor tyrosine kinase of the Met family. Receptor for macrophage stimulating protein. Can be desensitized by c-Cbl and its binding partner Grb2. Overexpression of wild-type RON causes the formation of lung tumors. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); TK group; Met family

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: cell surface; integral to plasma membrane; stress fiber

Molecular Function: macrophage colony stimulating factor receptor activity; protein binding; enzyme binding; ATP binding

Biological Process: positive regulation of MAP kinase activity; positive regulation of protein kinase B signaling cascade; peptidyl-tyrosine phosphorylation; multicellular organismal development; response to virus; single fertilization; positive regulation of cell proliferation; innate immune response; defense response; signal transduction; cell motility; transmembrane receptor protein tyrosine kinase signaling pathway